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- Clone
- 1H10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MACH, FLICE, Mch5
- Isotype
- Rat IgG2a
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot analysis of cell lysates from HeLa cells, untreated (-) or treated with Staurosporine (+, 1 µM, 8 hours), using Caspase-8 (Clone 1H10, 1:1000 dilution, 0.5 µg/ml) rat primary antibody and HRP Goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
Caspase-8 is involved in the apoptosis mediated by CD95 and TNFR-1. It is suggested that following the triggering of apoptosis by CD95 or TNFR-1, the adaptor molecule FADD interact physically with cytosolic caspase-8 and initiate the apoptotic cascade. The active caspase-8 consists of two subunits of p18 and p10. This antibody should recognize the whole length of human caspase-8 and some isofrms of lower MW.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length caspase-8 fusion protein
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC, ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-1 µg antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Sanchez I et al. 1999. Neuron. 22:623
- Li H, et al. 1998. Cell 94:491
- Product Citations
-
- RRID
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AB_2068466 (BioLegend Cat. No. 645501)
Antigen Details
- Structure
- Full length protein of 55kd. Contains two homologous domains of DED (death effector domain) of FADD. The remainder of the protein is similar to the CED-3 subfamily of caspases.
- Distribution
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Expressed in multiple isoforms in multiple tissues. Relatively high level in peripheral leucocytes.
- Function
- Caspase-8 is involved in apoptosis mediated by CD95 and TNFR-1.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
- Antigen References
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1. Muzio M et al. 1996. Cell 817-827
2. Cohen GM. 1997. Biochem J 326:1 - Gene ID
- 841 View all products for this Gene ID
- UniProt
- View information about Caspase-8 on UniProt.org
Related FAQs
Other Formats
View All Caspase-8 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Caspase-8 | 1H10 | WB,IHC,ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Caspase-8
Western blot analysis of cell lysates from HeLa cells, untre...
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