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Purified anti-CD102 (ICAM-2) Antibody

Pricing & Availability
Clone
W22039C (See other available formats)
Regulatory Status
RUO
Other Names
Intercellular Adhesion Molecule-2, ICAM-2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
W22039C_PURE_CD102_Antibody_1_013124
Human whole blood after RBC lysis was stained with Purified anti-CD102 (ICAM-2) (clone W22039C) (filled histogram) or Purified Mouse IgG2b, κ Isotype Control (dashed histogram, negative control) (Cat. No. 400302) followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (panel A). As a comparison to another BioLegend antibody clone against CD102 (ICAM-2), we stained the same starting sample with Purified anti-human CD102 (ICAM-2) (clone CBR-IC2/2) (Cat. No. 328502) or Purified Mouse IgG2a, κ Isotype Control (dashed histogram, negative control) (Cat. No. 400202) followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (panel B).
  • W22039C_PURE_CD102_Antibody_1_013124
    Human whole blood after RBC lysis was stained with Purified anti-CD102 (ICAM-2) (clone W22039C) (filled histogram) or Purified Mouse IgG2b, κ Isotype Control (dashed histogram, negative control) (Cat. No. 400302) followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (panel A). As a comparison to another BioLegend antibody clone against CD102 (ICAM-2), we stained the same starting sample with Purified anti-human CD102 (ICAM-2) (clone CBR-IC2/2) (Cat. No. 328502) or Purified Mouse IgG2a, κ Isotype Control (dashed histogram, negative control) (Cat. No. 400202) followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (panel B).
  • W22039C_PURE_CD102_Antibody_2_013124
    HUVEC cells (positive cell line, panel A) and HeLa cells (negative cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes followed by permeabilization with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 10 µg/mL of Purified anti-CD102 (ICAM-2) (clone W22039C), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 50 µm
  • W22039C_PURE_CD102_Antibody_3_013124
    Whole cell extracts (15 µg total protein) from indicated cell lines were incubated at 37°C for 30 minutes and were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of Purified anti-CD102 (ICAM-2) (clone W22039C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Save
631351 25 µg ¥39,600
631352 100 µg ¥99,000
Description

CD102 (ICAM-2) is a 55 kD type I transmembrane glycoprotein which belongs to the immunoglobulin superfamily. It is also known as Intercellular Adhesion Molecule-2, or ICAM-2. CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation, and in co-stimulation of the immune response. CD102 and CD54 in endothelial cells, such as in blood-brain barrier, are essential for assisting T cell polarization and crawling and diapedesis. CD102 is a counter receptor of LFA-1 (CD11a/CD18), DC-SIGN and MAC-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD102 (ICAM-2) recombinant protein fragment
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For western blotting (WB) it is recommended to heat the cell lysate at 37 °C for 30 min rather than boiling it.

For immunocytochemistry (ICC), either Fixation Buffer (Cat. No. 420801) or 4% paraformaldehyde followed by methanol permeabilization is recommended.

The reactivity of this antibody (clone W22039C) in cell surface flow cytometry is able to be blocked by another BioLegend antibody (clone CBR-IC2/2) (Cat. No. 328502).

RRID
AB_3097547 (BioLegend Cat. No. 631351)
AB_3097547 (BioLegend Cat. No. 631352)

Antigen Details

Structure
55 kD type I transmembrane glycoprotein, Ig superfamily
Distribution

Vascular endothelial cells, lymphocytes, monocytes, platelets

Function
Adhesion, costimulation
Ligand/Receptor
LFA-1, DC-SIGN, MAC-1
Cell Type
Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Weber KSC, et al. 2004. Inflammation. 28:177-88.
  2. Barclay AN, et al. 1997. The Leukoycte Antigen Factsbook.
  3. Casasnovas JM, et al. 1999. Proc Natl Acad Sci USA. 96:3017-22.
  4. Diamonds MS, et al. 1990. J Cell Biol. 111:3129-39.
  5. Marchetti L & Engelhardt B. 2020. Vasc Biol. 2:H1-H18.
Gene ID
3384 View all products for this Gene ID
UniProt
View information about CD102 on UniProt.org
Go To Top Version: 1    Revision Date: 01/31/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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