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Purified anti-CD133 (Prominin-1) Antibody

Pricing & Availability
Clone
8F2-H6-D6 (See other available formats)
Regulatory Status
RUO
Other Names
Prom1, Prominin-1, CD133
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
8F2-H6-D6_Purified_CD133_Antibody_040119
Western blot of purified anti-CD133 (Prominin-1) antibody (clone 8F2-H6-D6). Lane 1: Molecular weight marker; Lane 2: 30 µg of human liver lysate; Lane 3: 40 µg of human brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 8F2-H6-D6_Purified_CD133_Antibody_040119
    Western blot of purified anti-CD133 (Prominin-1) antibody (clone 8F2-H6-D6). Lane 1: Molecular weight marker; Lane 2: 30 µg of human liver lysate; Lane 3: 40 µg of human brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Save
858601 25 µg ¥23,320
858602 100 µg ¥60,720
Description

Prominin-1 (Prom1), also known as CD133, is a transmembrane glycoprotein located mainly on cellular protrusions of neurons and glial stem cells in the nervous system. CD133 level has been used as marker for isolation of cancer stem cells from various gliomas. Prom1 is also expressed in other tissues including kidney, mammary glands, and uterus.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801221 (BioLegend Cat. No. 858601)
AB_2801221 (BioLegend Cat. No. 858602)

Antigen Details

Structure
Prom1 is an 865 amino acid protein with an apparent molecular mass of ~ 97 kD.
Distribution

Cellular Distribution: Plasma membrane, extracellular, and endoplasmic reticulum.
Tissue Distribution: Glioblastomas; liver,gallbladder, cervix, uterus, and endometrium.

Function
Used as a marker for isolation of cancer stem cells.
Interaction
CDHR1, Actin, NAT8, NAT8B
Cell Type
Neural Stem Cells, Neurons
Biology Area
Immuno-Oncology, Neuroscience
Molecular Family
CD Molecules
Antigen References
  1. Nagare RP, et al. 2017. Curr Stem Cell Res Ther. 12(1):37
  2. Dahlrot RH, et al. 2013. Int J Clin Exp Pathol. 6(3):334
  3. Donovan LK, et al. 2012. Cell Prolif. 45(6):527
Gene ID
8842 View all products for this Gene ID
UniProt
View information about CD133 on UniProt.org
Go To Top Version: 1    Revision Date: 04/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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