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Purified anti-CD2AP Antibody

Pricing & Availability
Clone
W21094A (See other available formats)
Regulatory Status
RUO
Other Names
Adapter protein CMS, AL024079, C78928, Cas ligand with multiple SH3 domains, CD2 associated protein, CMS, Mesenchyme to epithelium transition protein with SH3 domains 1, METS 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W21094A_PURE_CD2AP_WB_101723
Whole cell extracts (15 µg total protein) from the indicated cell lines (lane 1 and 2 are A431 cells without and with CD2AP knockdown, respectively) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of Purified anti-CD2AP (clone W21094A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W21094A_PURE_CD2AP_WB_101723
    Whole cell extracts (15 µg total protein) from the indicated cell lines (lane 1 and 2 are A431 cells without and with CD2AP knockdown, respectively) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of Purified anti-CD2AP (clone W21094A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W21094A_PURE_CD2AP_ICC_101723
    A431 cells without (panel A and B) and with (panel C and D) CD2AP knockdown were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5.0 µg/mL of Purified anti-CD2AP (clone W21094A), followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 Goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature (panel A and panel C). Nuclei were counterstained with DAPI (Cat. No. 422801) (panel B and D), and the image was captured with a 40X objective. Scale bar: 25 µm
  • C. W21094A_PURE_CD2AP_IHCP_101723
    IHC staining using Purified anti-CD2AP (clone W21094A) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Citrate buffer (Cat. No. 420901), the tissue was incubated with 5 µg/mL of Purified anti-CD2AP (clone W21094A) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (red) (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Save
625151 25 µg ¥36,300
625152 100 µg ¥90,200
Description

CD2‐associated protein (CD2AP) is a scaffolding molecule connecting membrane proteins to the cytoskeleton. CD2AP also participates in the immunological synapse between CD2-expressing T cells and antigen-presenting cells. T cell activation induces cell adhesion through CD2-mediated binding of antigen-presenting cells to surface ligands. This enhances antigen-specific T cell activation, cell clustering, and induces cytoskeletal polarization. The interactions between CD2AP and other cytoskeletal proteins can regulate EGFR endocytosis. CD2AP is expressed at highest levels in liver, thymus, and spleen. Ablation of CD2AP disrupted the tissue architecture of the lamina propria cells of the gastric mucosa, indicating that CD2AP plays an important role in maintaining the normal morphology of the gastric mucosa. CD2AP-deficient mice develop a fatal congenital nephrotic syndrome, suggesting that CD2AP is also involved in maintaining renal glomerular integrity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human CD2AP protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The clone does not cross-react with mouse in western blotting (WB).

For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice-cold methanol is recommended.

For immunohistochemistry (IHC-P), antigen retrieval buffer Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 is recommended.

Additional Product Notes

This antibody has been tested in knockout/knockdown models for Western Blotting and Immunocytochemistry.

RRID
AB_3097249 (BioLegend Cat. No. 625151)
AB_3097249 (BioLegend Cat. No. 625152)

Antigen Details

Structure
CD2AP is a 639 amino acid protein with a predicted molecular weight of 71 KD
Distribution

Widely expressed in fetal and adult tissues

Function
Regulating signal transduction and cytoskeletal molecules
Interaction
F-actin, PKD2, NPHS1, NPHS2, WTIP, DDN, CBL, BCAR1/p130Cas, MVB12A, ARHGAP17, ANLN, CD2, CBLB, PDCD6IP, TSG101, RIN3, CGNL1 and SH3BP1
Cell Type
Neurons, T cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience
Molecular Family
Adaptor Proteins, APP/β-Amyloid
Antigen References
  1. Kirsch KH, et al. 1999. EMBO J. 96:6211-16.
  2. Kirsch KH, et al. 2001. J Biol Chem. 276:4957-63.
  3. Lynch DK, et al. 2003. J. Biol. Chem. 278:21805-13.
  4. Hutchings NJ, et al. 2003. J. Biol. Chem. 278:22396-403.
  5. Shih NY. et al. 1999. Science. 286:312-15.
Gene ID
23607 View all products for this Gene ID
UniProt
View information about CD2AP on UniProt.org
Go To Top Version: 1    Revision Date: 10/13/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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