- Clone
- W23186F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-cell surface glycoprotein CD3 epsilon chain, T3
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
649951 | 25 µg | ¥24,200 | |
649952 | 100 µg | ¥60,500 |
The CD3ε chain of the T-cell receptor (TCR)-CD3 complex is a type I membrane protein that plays a critical role in antigen recognition, TCR signal transduction and T-cell activation following binding to the peptide/MHC antigen complex. CD3ε is expressed by all mature T cells and forms a heteromeric complex with CD3 γ, δ and ζ chains, and either α/β or γ/δ chains of the TCR heterodimer. Upon activation, CD3 undergoes a conformational change, leading to exposure of the proline rich sequence in CD3ε and recruitment of Lck. CD3 is also important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of the intracellular domain of human CD3ε
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).
For use in intracellular flow cytometry (ICFC), fixation/permeabilization with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) is recommended. Permeabilization with True-Phos™ Perm Buffer is not recommended.
This antibody (clone W23186F) is not recommended for use in immunocytochemistry (ICC) due to dim signal.
Antigen Details
- Structure
- CD3ε is a 207 amino acid protein with a predicted molecular weight of 23 kD.
- Distribution
-
Plasma membrane
- Function
- Co-receptor
- Interaction
- CD3ε forms a heteromeric complex with CD3 γ, δ.and ζ chains, and either α/β or γ/δ chains of the TCR heterodimer.
- Ligand/Receptor
- CD3ε, as part of the TCR-CD3 complex, recognizes fragments of antigens bound to major histocompatibility complex molecules
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
- Schlossman S, et al. 1995. Leucocyte Typing V Oxford University Press New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Sedelies KA, et al. 2004. J Biol Chem. 279:26581-7.
- Rivollier A, et al. 2004. Blood. 104:4029-37.
- Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860-73.
- Smeltz RB. 2007. J Immunol. 178:4786-92.
- Gene ID
- 12501 View all products for this Gene ID
- UniProt
- View information about CD3epsilon on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD3ε Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CD3ε | W23186F | IHC-P,ICC,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-CD3ε
IHC staining with Purified anti-CD3ε (clone W23186F) on form... IHC staining with Purified anti-CD3ε (clone W23186F) on form... Whole cell extracts (15 µg total protein per lane) from the ... Whole cell extracts (15 µg total protein per lane) from mous... Human peripheral blood lymphocytes were fixed and permeabili...
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