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Purified anti-CD44 Antibody

Pricing & Availability
Clone
M588 (See other available formats)
Regulatory Status
RUO
Other Names
CD44, CD44 Molecule, LHR, CD44 Antigen, Cell Surface Glycoprotein CD44, CDW44
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_M588_Pure_CD44_Antibody_1_030719
Western blot of purified anti-CD44 antibody (clone M588). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 10 µg of HeLa cell lysate; Lane 4: 30 µg of mouse Brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A_M588_Pure_CD44_Antibody_1_030719
    Western blot of purified anti-CD44 antibody (clone M588). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 10 µg of HeLa cell lysate; Lane 4: 30 µg of mouse Brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • B_M588_Pure_CD44_Antibody_2_030719
    ICC staining of purified anti-CD44 antibody (clone M588) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 5 µg/ml of Alexa Fluor® 647 goat anti-Rat IgG for one hour at room temperature. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_M588_PURE_CD44_Antibody_052022
    IHC staining of anti-CD44 antibody (clone M588) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10.0 μg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm.
Cat # Size Price Save
858801 25 µg ¥23,320
858802 100 µg ¥60,720
Description

CD44 is a cell-surface glycoprotein that is expressed in a large number of cell types. CD44 functions as an adhesion molecule that is important for cell migration and intercellular interactions and adhesion. CD44 transcripts undergo complex alternative splicing, which leads to a variety of functionally distinctive isoforms of this protein. Transcription of CD44 has been shows to be partially activated by Wnt/beta catenin signaling pathway, and thus is linked to tumor development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 5.0 - 10 μg/mL is recommended. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801225 (BioLegend Cat. No. 858801)
AB_2801225 (BioLegend Cat. No. 858802)

Antigen Details

Structure
CD44 is a 742 amino acid protein with an apparent molecular mass of ~ 82 kD.
Distribution

Tissue distribution: Ubiquitously expressed in all tissues.
Cellular Distribution: Plasma membrane, extracellular, cytosol, and golgi apparatus.

Function
Intercellular interaction and cell adhesion,cellular migration
Interaction
HA, glycosaminoglycans, collagen, laminin, fibronectin, UNC119, PDPN
Ligand/Receptor
Receptor for Hyaluronate
Antigen References
  1. Price ZK, et al. 2018. Cancers (Basel). 10(12): E482
  2. Ouhtit A, et al. 2018. Int J Biol Sci. 14(13): 1782
  3. Mooney KL, et al. 2016. J Clin Neurosci. 34:1
Gene ID
960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org
Go To Top Version: 2    Revision Date: 05/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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