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Purified anti-CD73 Antibody

Pricing & Availability
Clone
AA60-E3-3 (See other available formats)
Regulatory Status
RUO
Other Names
NT5E, 5'-Nucleotidase Ecto, Ecto-5'-Nucleotidase, CALJA, ENT, NT5, EN, E5NT
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a. AA60-E3-3_PURE_CD73_WB_060623
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-CD73 (clone AA60-E3-3) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. AA60-E3-3_PURE_CD73_WB_060623
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-CD73 (clone AA60-E3-3) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. AA60-E3-3_PURE_CD73_ICC_060623
    HeLa cells (low expression control) (panel A) and U-87 MG cells (high expression control) (panel B) were fixed with ice-cold MeOH for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 2.5 µg/mL purified anti-CD73 (clone AA60-E3-3) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale: 50 µm
  • c. AA60-E3-3_PURE_CD73_ICFC_060623
    U-87 MG cells (filled histogram, positive cell line) and HeLa cells (open histogram, negative cell line) were stained with anti-CD73 (clone AA60-E3-3) or purified mouse IgG1, κ isotype control (Cat. No. 401402) (dashed, open histogram) followed by PE goat anti-mouse IgG (Cat. No. 405307).
Cat # Size Price Save
614951 25 µg ¥26,400
614952 100 µg ¥86,900
Description

CD73 (cluster of differentiation 73), also known as ecto-5’-nucleotidase (e5NT), is a membrane-bound glycoprotein and ectoenzyme. CD73 catalyzes the hydrolysis of AMP generated by CD39 to adenosine to activate adenosine signaling. This role makes CD73 crucial to the regulation of purinergic signaling and immune homeostasis and response. CD73 also serves a variety of physiological functions, including lymphocyte binding, epithelial ion transport, ischemic preconditioning, tissue-barrier function, T cell activation, and tissue homeostasis. Overexpressed in several cancers and implicated in tumor progression, metastasis, and the impairment of anti-tumor immune response via adenosine receptor (AR) signaling, CD73 is a target of interest in cancer therapy. It is also a potential immunotherapy target for the treatment of pain, inflammation, and hypoxia.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human CD73
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not mouse or rat reactive. 

This clone is not suitable for use in immunohistochemistry.

For use in ICC, we recommend fixation with ice-cold methanol or fixation with PFA followed by permeabilization with ice-cold methanol.

RRID
AB_2941621 (BioLegend Cat. No. 614951)
AB_2941621 (BioLegend Cat. No. 614952)

Antigen Details

Structure
CD73 is a 574 amino acid protein with a predicted molecular weight of 63 kD.
Distribution

Cytoplasmic and membranous expression in most tissues

Function
Hydrolase, metabolic protein, CD marker, enzyme
Interaction
AP-2, SMAD, SP-1, CD39, GSDMD
Ligand/Receptor
Metal-binding, nucleotide-binding, zinc
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology
Molecular Family
CD Molecules, Enzymes and Regulators, Innate Immune Signaling
Antigen References
  1. Knapp K, et al. 2012. Structure. 20:2161-73.
  2. Minor M, et al. 2019. Am J Physiol Cell Physiol. 317:C1079-C1092.
  3. Yu M, et al. 2020. Nat Commun. 11:515.
Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org
Go To Top Version: 1    Revision Date: 06/06/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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