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- Clone
- A18119A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- complement C5 beta chain, C5a
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-Complement C5b antibody (clone A18119A). Lane 1: Molecular weight marker; Lane 2: 10 µg of human plasma; Lane 3: 40 µg of HepG2 cell lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Complement C5b antibody (clone A18119A) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Save |
|---|---|---|---|
| 869901 | 25 µg | ¥23,320 | |
| 869902 | 100 µg | ¥60,720 |
The complement system is a part of eukaryotic innate defense system that enhances the clearance of exogenous microbes and damaged cells by antibodies and phagocytic cells. In vivo, the complement system is activated by three pathways: the classical complement pathway, the alternative complement pathway, and the lectin pathway. Complement C5 is a 1676 amino acid precursor protein synthesized in the liver. All three complement activation pathways culminate in the cleavage of C5, which produces two disulfide linked glycoproteins C5α and C5β. C5α is further cleaved by C5 convertase into C5a and C5α', the latter binds to C5β to form C5b, which in turn sequentially recruits C6-C9 to form the membrane attack complex (MAC). MAC destroys pathogens by forming a lytic pore in their membrane. Mutations affecting the Complement C5 gene lead to complement component 5 deficiency where patients demonstrate an increased propensity to develop severe recurrent infections.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acids 428–454 of human complement C5 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody predominantly detects C5b in human cell line and human plasma. At high concentration (between 2 and 10 µg/mL), the full-length C5 is also detected at ~190 kD in human cell lines, but not in human plasma.
- RRID
-
AB_2820182 (BioLegend Cat. No. 869901)
AB_2820182 (BioLegend Cat. No. 869902)
Antigen Details
- Structure
- C5b is a 655 amino acid protein with predicted and observed molecular mass of ~ 75 kD.
- Distribution
-
Tissue Distribution: Predominantly in liver and plasma. Also expressed in the brain.
Cellular Distribution: Plasma membrane and extracellular - Function
- Complement C5b generation and assembly of MAC facilitates the lysis and destruction of pathogens.
- Interaction
- Interacts with C5a chain
- Ligand/Receptor
- Staphylococus aureus protein SSL5, binds C6 (transiently)
- Cell Type
- Astrocytes, Mature Neurons, Microglia
- Biology Area
- Cell Biology, Complement, Neurodegeneration, Neuroinflammation, Neuroscience
- Molecular Family
- Innate Immune Signaling
- Antigen References
-
- Vlaicu SI, et al. 2019. Front Immunol. 10:1054
- Fishelson Z, et al. 2019. Front Immunol. 10:752
- Bergseth G, et al. 2013. Mol Immunol. 56(3):232
- Gene ID
- 727 View all products for this Gene ID
- UniProt
- View information about Complement C5b on UniProt.org
Other Formats
View All Complement C5b Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Complement C5b | A18119A | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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