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Purified anti-CREB1 Phospho (Ser133) Antibody

Pricing & Availability
Clone
Poly22A03 (See other available formats)
Regulatory Status
RUO
Other Names
Cyclic AMP-Responsive Element-Binding Protein 1 (Serine 133), CAMP Responsive Element Binding Protein 1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly22A03_PURE_CREB1_Antibody_051623
Whole cell extracts (15 µg protein) from serum-starved HeLa cells untreated (-) or treated (+) with 25 µg/mL Anisomycin for 30 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-CREB1 Phospho (Ser133) (clone Poly22A03) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution. Equal CREB1 loading was confirmed by probing membranes with anti-pan CREB1 antibody. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • Poly22A03_PURE_CREB1_Antibody_051623
    Whole cell extracts (15 µg protein) from serum-starved HeLa cells untreated (-) or treated (+) with 25 µg/mL Anisomycin for 30 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-CREB1 Phospho (Ser133) (clone Poly22A03) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution. Equal CREB1 loading was confirmed by probing membranes with anti-pan CREB1 antibody. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Save
611651 50 µL ¥67,100
611652 200 µL ¥154,000
Description

Cyclic AMP (cAMP)-response element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor that binds to the cAMP response element (CRE) in response to certain stimuli. CREB1 contains a basic leucine zipper (bZIP) DNA-binding domain, 1 dimerization domain, and 9 serine residues in 1 kinase inducible domain phosphorylated by multiple kinases, including Ca2+/CaM-dependent kinase (CaMK) II and IV, protein kinase A (PKA), protein kinase C (PKC), mitogen/stress-activated kinase (MSK), ribosomal S6 kinase (RSK), AKT, and MAPKAP kinase 2 (MK2). Both the protein levels and activation of CREB1 are vital for maintaining cellular homeostasis in physiological and pathological conditions. Phosphorylation of CREB1 at Ser133 promotes the recruitment of co-activator proteins CBP and p300, which has been proposed to increase transcription of CREB-dependent genes. Ser133 can also be phosphorylated by other proteins such as MSK1/2 in response to the activation of MAPK signaling.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Synthetic peptide of human CREB phosphorylated at Ser133
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:2000-1:3000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The CREB1 peptide phosphorylated at Serine 133 used for immunization has 100% homology with ATF-1 sequence phosphorylated at serine 63. Therefore, this clone will detect both phosphorylated proteins if present in testing sample.

RRID
AB_3083400 (BioLegend Cat. No. 611651)
AB_3083400 (BioLegend Cat. No. 611652)

Antigen Details

Structure
CREB1 contains 327 amino acids at a predicted molecular weight at 43 kD
Distribution

Ubiquitously expressed/Nucleus

Function
Under physiological conditions, CREB1 maintains cellular survival, proliferation, anti-inflammatory immune responses and modulates the development of the nervous system by serving a neuroprotective role. Under pathological conditions, increased levels of CREB1 is associated with acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin’s lymphoma, chronic lymphatic leukemia (CLL), melanoma, hepatocellular, renal cell, ovarian, prostate, lung, gastric, esophageal, pancreatic, and breast tumors. CREB1 is also involved in the aging process by regulating the downstream signals of mitochondria dysfunction.
Interaction
CREB1 interacts with CREBBP, CRTC2, EP300, CRTC1, JUN, AKT1, CAMK4, FOS, RPS6KA1, RPS6KA3, CaMKII, CaMKIV, PKA, PKC, MSK, RSK, AKT, and MK2.
Cell Type
B cells, Endothelial cells, Fibroblasts, Granulocytes, Macrophages, NK cells, T cells
Biology Area
Cell Biology, Transcription Factors
Antigen References
  1. Andre S, et al. 2020. Cell Mol Life Sci. 77:4049-4067.
  2. Pei-Hsuan L, et al. 2020. Diagnostics. 10:295.
  3. Kensuke S, et al. 2011. J Neurochem. 116:1-9.
  4. Andy YW, et al. 2010. J Immunol. 185:6413-6419.
  5. Arnould T, et al. 2002. EMBO J. 21:53-63. 
  6. Shaista N, et al. 2014. Biochem J. 458:469-79.
Gene ID
1385 View all products for this Gene ID
UniProt
View information about CREB1 Phospho Ser133 on UniProt.org

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Go To Top Version: 1    Revision Date: 05/17/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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