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Purified anti-CRMP5 Antibody

Pricing & Availability
Clone
A18231D (See other available formats)
Regulatory Status
RUO
Other Names
Collapsin Response Mediator Protein 5, Dihydropyrimidinase Like 5, DPYSL5, DRP-5, UNC33-Like Phosphoprotein 6, Ulip6
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A18231D_PURE_CRMP5_-IHC-P_033023
IHC staining of purified anti-CRMP5 (clone A18231D) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Tris-EDTA pH 9.0 buffer (10 mM Tris, 1 mM EDTA), the tissue was incubated with (panel A) and without (panel B) 5.0 µg/mL of purified anti-CRMP5 (clone A18231D), followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 20 µm
  • A. A18231D_PURE_CRMP5_-IHC-P_033023
    IHC staining of purified anti-CRMP5 (clone A18231D) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Tris-EDTA pH 9.0 buffer (10 mM Tris, 1 mM EDTA), the tissue was incubated with (panel A) and without (panel B) 5.0 µg/mL of purified anti-CRMP5 (clone A18231D), followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 20 µm
  • B. A18231D_PURE_CRMP5_-WB_033023
    Whole cell extracts (15 µg total protein) from the indicated cell line treated with siRNA control (-) or CRMP5 siRNA (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-CRMP5 (clone A18231D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Save
614551 25 µg ¥31,900
614552 100 µg ¥91,300
Description

CRMP5 (Collapsin Response Mediator Protein 5 or DPYSL5) is a member of the CRMP family and is involved in regulation of the cytoskeleton. CRMP5 acts as a negative regulator of microtubule stability and dynamics and participates in the fibroblast growth factor (FGF) signaling pathway, regulating axon guidance and neuronal development. CRMP5 has been implicated in several human diseases. In cancer cells, CRMP5 regulates cancer cell migration, invasion, and proliferation through its interaction with the tumor suppressor protein, PTEN. Mutations in CRMP5 are related to neurodevelopmental disorders, including X-linked intellectual disability and autism spectrum disorder. CRMP5 regulates tau protein degradation through its interaction with microtubules and enzymes, suggesting its role in neuroinflammation and Alzheimer's disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of CRMP5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for use in immunocytochemistry (ICC).

For use in immunohistochemistry (IHC), antigen retrieval with Tris-EDTA pH 9.0 is recommended.

RRID
AB_2941617 (BioLegend Cat. No. 614551)
AB_2941617 (BioLegend Cat. No. 614552)

Antigen Details

Structure
Forms homotetramer and heterotetramer with the other CRMP proteins
Distribution

Cytoplasm; expressed in Neuronal tissue and lung

Function
Regulate cytoskeleton stability and dynamics; axon guidance
Interaction
Interact with other CRMP proteins, MAP2, TUBB3
Cell Type
Mature Neurons, Neural Stem Cells, Neurons
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Neurodegeneration, Neuroinflammation, Neuroscience, Synaptic Biology
Molecular Family
APP/Aβ Degradation, Microtubules, Tau
Antigen References
  1. Yu Z, et al. 2001. Ann Neurol. 49:146-54.
  2. Bretin S, et al. 2005. J Comp Neurol. 486:1-17.
  3. Yamashita N, et al. 2011. J Neurosci. 31:1773-9.
  4. Lin YS, et al. 2019. Neuropharmaology. 157:107673.
Gene ID
56896 View all products for this Gene ID
UniProt
View information about CRMP5 on UniProt.org
Go To Top Version: 1    Revision Date: 03/30/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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