IHC staining of purified anti-Cytokeratin antibody (clone AE-1) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-Cytokeratin antibody (clone AE-1) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-Cytokeratin antibody (clone AE-1) on formalin-fixed paraffin-embedded dog skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-Cytokeratin antibody (clone AE-1) on formalin-fixed paraffin-embedded primate skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
This antibody recognizes low and intermediate molecular weight type I acidic keratins (40, 48, 50, and 56.5 kD). This clone stains the basal layer of epidermis and most epithelia.
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References
(PubMed link indicates BioLegend citation)
Yang K, et al. 1990. Amer. J. of Clin. Path. 94:261. (IHC) PubMed
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