Purified anti-Cytokeratin Antibody

Pricing & Availability
Clone
AE-3 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
AE-3_Purified_Cytokeratin_Antibody_1_013019.png
IHC staining of purified anti-Cytokeratin antibody (clone AE-3) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 2.8 µg/ml of the primary antibody for 60 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • AE-3_Purified_Cytokeratin_Antibody_1_013019.png
    IHC staining of purified anti-Cytokeratin antibody (clone AE-3) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 2.8 µg/ml of the primary antibody for 60 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • AE-3_Purified_Cytokeratin_Antibody_2_013019.png
    IHC staining of purified anti-Cytokeratin antibody (clone AE-3) on formalin-fixed paraffin-embedded rat skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 2.8 µg/ml of the primary antibody for 60 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • AE-3_Purified_Cytokeratin_Antibody_3_013019.png
    IHC staining of purified anti-Cytokeratin antibody (clone AE-3) on formalin-fixed paraffin-embedded dog skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 2.8 µg/ml of the primary antibody for 60 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • AE-3_Purified_Cytokeratin_Antibody_4_013019.png
    IHC staining of purified anti-Cytokeratin antibody (clone AE-3) on formalin-fixed paraffin-embedded primate skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 2.8 µg/ml of the primary antibody for 60 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Save
914104 100 µg ¥50,380
Description

This antibody recognizes high molecular weight keratins (52, 54, 56, 58, 59, 64 and 65-67 kD) of the Type II basic subfamily. This clone stains the entire epidermis (basal layer and above) and most epithelia.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Reported Reactivity
Human, Mouse, Dog, Non-Human Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using human epidermal keratins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2616959 (BioLegend Cat. No. 914104)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Cytokeratin on UniProt.org
Go To Top Version: 3    Revision Date: 08/16/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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