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- Clone
- 34βE12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cytokeratin, high-molecular weight keratin (HMWK), high-molecular weight cytokeratin (HMWCK)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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IHC staining of anti-Cytokeratin, HMW antibody (clone 34βE12) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody at 0.7 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
This antibody is specific for high molecular weight cytokeratins 1, 5, 10 and 14. Clone 34βE12 is immunoreactive with squamous and adeno-squamous carcinomas. In normal epithelia, the antibody stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This antibody is also useful in differentiating benign and malignant tumors of the prostate gland.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was developed using a solubilized keratin extracted from human stratum corneum.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/ml is suggested. For Western blotting, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The Cytokeratin, High Molecular Weight (HMW) antibody (clone 34ßE12) reacts primarily with complex epithelium such as squamous, ductal and transitional epithelium and corresponding tumors.
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Application References
(PubMed link indicates BioLegend citation) -
- Gown AM, Vogel AM. 1985. Am. J. Clin. Pathol. 84:413. (IHC)
- Gown AM, Vogel AM. 1982. J. Cell Biol. 95:414. (IHC)
- RRID
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AB_2629548 (BioLegend Cat. No. 916504)
Antigen Details
- Distribution
-
Tissue distribution: skin, prostate or squamous cell carcinoma.
Cellular distribution: cytoplasm. - Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- NA
- UniProt
- View information about Cytokeratin on UniProt.org
Related FAQs
Other Formats
View All Cytokeratin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Cytokeratin, HMW | 34βE12 | IHC-P,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Cytokeratin, HMW
IHC staining of anti-Cytokeratin, HMW antibody (clone 34βE12...
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