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- Clone
- O94A3 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-Doublecortin (DCX) antibody (clone O94A3). Lane 1: Molecular weight marker; Lane 2: 10 µg of embryonic mouse brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 868501 | 25 µg | ¥23,320 | |
| 868502 | 100 µg | ¥60,720 |
Doublecortin (DCX) is a microtubule-associated protein that is predominantly expressed in embryonic brains by neuronal precursor cells or immature neurons. DCX is also expressed in the adult brains in regions such as subventricular zones and dentate gyrus, where neuronal precursor cells are localized, and thus has been widely used as a marker for neurogenesis. Doublecortin binds to the microtubule cytoskeleton and stabilizes microtubule bundles. Mutation in DCX has been identified in diseases such as X-linked lissencephaly and double cortex syndrome.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human recombinant DCX protein produced in HEK293T cell.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2814620 (BioLegend Cat. No. 868501)
AB_2814620 (BioLegend Cat. No. 868502)
Antigen Details
- Structure
- DCX is a 366 amino acid protein with predicted and observed molecular mass of ~41 kD
- Distribution
-
Tissue Distribution: Brain
Cellular Distribution: Cytoskeleton, cytosol, and nucleus - Function
- DXC is a markers for neurogenesis. It binds to the microtubule cytoskeleton and stabilizes microtubule bundles.
- Interaction
- Tubulin, USP9X
- Cell Type
- Neural Stem Cells, Neurons
- Biology Area
- Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Cytoskeletal Proteins, Microtubules
- Antigen References
-
- Ayanlaja AA, et al. 2017. Front Mol Neurosci. 10:199
- Reiner O. 2013. Scientifica (Cairo). 2013:393975
- Reiner O, et al. 2004. Cell Cycle. 3(6):747
- Gene ID
- 13193 View all products for this Gene ID
- UniProt
- View information about Doublecortin on UniProt.org
Other Formats
View All Doublecortin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Doublecortin (DCX) | O94A3 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Doublecortin (DCX)
Western blot of purified anti-Doublecortin (DCX) antibody (c...
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