- Clone
- NT63 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
- Isotype
- Mouse IgG2a
- Ave. Rating
- Submit a Review
- Product Citations
- publications
RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the rpoB gene. RNA polymerase β is a subunit which, along with α (rpoC), limits core polymerase expression.
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified protein.
- Formulation
- Phosphate-buffered solution, pH 7.2.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
ELISA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.00625 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone NT63 recognizes epitope 922-1090.
-
Application References
(PubMed link indicates BioLegend citation) -
- Thompson NE, et al. 1992. Biochemistry 31:7003.
- Product Citations
-
- RRID
-
AB_2565555 (BioLegend Cat. No. 663006)
Antigen Details
- Structure
- 1252 amino acids with a predicted molecular weight of approximately 150 kD.
- Distribution
-
Bacteria.
- Function
- Transcriptional regulator in bacteria.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerard Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature 451:851. - Gene ID
- 948488 View all products for this Gene ID
- UniProt
- View information about RNA Polymerase beta on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All RNA Polymerase β Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-E. coli RNA Polymerase β | NT63 | WB,ELISA |
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Purified anti-E. coli RNA Polymerase β
Total lysates (15 µg protein) from E. coli BL21 cells were r... Total cell lysate from E Coli. Strain BL21 (15 µg protein) w...
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