- Clone
- NT73 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNA pol beta prime, rpoC, tabB, DNA-directed RNA polymerase subunit beta prime, RNAP subunit beta prime, RNA polymerase subunit beta prime, Transcriptase subunit beta prime
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the rpoB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Reported Reactivity
- Other species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- 1295-1417 (SLAELLNAGLGGS) of the E. coli RNA polymerase β subunit.
- Formulation
- Phosphate-buffered solution, pH 7.2.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
ELISA, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone NT73 is extremely useful for purifying holoenzymes from enterics. It does not cross-react with Mycobacterial smegmatis RNAP. This antibody is used for purifying Softag 1 epitope tagged protein expressed in eukaryotic systems. Clone NT73 recognizes epitope 1295-1417 (SLAELLNAGLGGS).
Additional reported applications (for the relevant formats) include: immunoprecipitation. -
Application References
(PubMed link indicates BioLegend citation) -
- Thompson NE, et al. 1992. Biochemistry 31:7003.
- Thompson NE, et al. 2003. Anal. Biochem. 323:171.
- Product Citations
-
- RRID
-
AB_2566242 (BioLegend Cat. No. 662904)
Antigen Details
- Structure
- 1252 amino acids with a predicted molecular weight of approximately 150 kD.
- Distribution
-
Bacteria
- Function
- Transcriptional regulator in bacteria.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Hurwitz J, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature 451:851. - Gene ID
- 948488 View all products for this Gene ID
- UniProt
- View information about RNA Polymerase beta Prime on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All RNA Polymerase β Prime Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-E. coli RNA Polymerase ImmunoAffinity Resin | NT73 | IP |
Purified anti-E. coli RNA Polymerase β Prime | NT73 | WB,ELISA,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Anti-E. coli RNA Polymerase ImmunoAffinity Resin
-
Purified anti-E. coli RNA Polymerase β Prime
Total lysates (15 µg protein) from HeLa (lane 1) and E. coli... Total cell lysate from Hela cells (lane 1) and E Coli...
Follow Us