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Purified anti-E. coli RNA Sigma 32 Antibody

Pricing & Availability
Clone
3RH3 (See other available formats)
Regulatory Status
RUO
Other Names
SigmaH, rpoH, fam, hin, htpR, Heat shock regulatory protein F33.4, RNA Polymerase
Isotype
Mouse IgG1
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Product Citations
publications
3RH3_purified_E-coli-32_Antibody_1_100818_updated
Western blot analysis of 15 µg cell lysates from DH5α cells. Samples were incubated at 37°C for 0, 5, 10, 15, and 40 minutes and then resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 2 µg/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • 3RH3_purified_E-coli-32_Antibody_1_100818_updated
    Western blot analysis of 15 µg cell lysates from DH5α cells. Samples were incubated at 37°C for 0, 5, 10, 15, and 40 minutes and then resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 2 µg/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • 3RH3_purified_E-coli-32_Antibody_2_100818_updated
    Western blot analysis of 15 µg cell lysates from DH5α and BL21 cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 5 µg/mL purified anti-Sigma 32 antibody, clone 3RH3, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
Cat # Size Price Save
663404 25 µg ¥22,220
663405 100 µg ¥55,660
Description

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters.

Product Details
Technical data sheet

Product Details

Verified Reactivity
E. coli
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 3RH3 recognizes epitope 1-25.

Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Lesley SA, et al. 1987. J. Biol. Chem. 262:5404.
RRID
AB_2750247 (BioLegend Cat. No. 663404)
AB_2750247 (BioLegend Cat. No. 663405)

Antigen Details

Structure
Sigma 32 (σ32) is a 32 kD transcription factor of E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947970 View all products for this Gene ID
UniProt
View information about Sigma 32 on UniProt.org
Go To Top Version: 1    Revision Date: 10/01/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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