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- Clone
- P83A7A8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Eukaryotic translation initiation factor 2A, 65 kD eukaryotic translation initiation factor 2A
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total cell or tissue lysates (15 µg protein) from SH-SY5Y, HepG2, OVCAR3 and mouse brain were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-eIF-2A antibody (clone P83A7A8). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control. -
Total cell lysates (15 µg protein) from Daudi (lane 1), THP-1 (lane 2), HeLa (lane 3) and NIH3T3 (lane 4) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-eIF-2A antibody (clone P83A7A8) (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-eIF-2A antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower blot). Lane M: Molecular weight ladder.
eIF-2A was initially considered to be a eukaryotic functional counterpart of the prokaryotic translation initiation factor IF2, because it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner. But recent studies proved that eIF2-mediated pathway is far more efficient, and only eIF2 is able to form the 80S initiation complex on native globin mRNA. eIF2 is responsible for the loading of Met-tRNAi onto the ribosome in the majority of translation initiation events in eukaryotes, while eIF-2A is involved in the translation of a limited subset of mRNAs.
Homologues of eIF-2A are found in a wide range of eukaryotic species, indicating that eIF-2A plays some conserved roles. eIF-2A was reported to be involved in antigen presentation by major histocompatibility complex (MHC) class I molecules. Some antigenic precursors are translated from CUG codons via an unknown mechanism, which is different from the conventional methionine-initiated mechanism. Ribosome profiling experiments also revealed that translation initiation at CUG or GUG codons is a common event in yeast and human, and is not limited to antigen presentation by MHC class I molecules. Under stress conditions, such as amino acid starvation, heat shock and viral infection, eIF-2A migrates from the nucleus to the cytoplasm, where it is utilized for the translation of alphavirus 26S mRNA and hepatitis C viral (HCV) mRNA. So eIF-2A is considered to be a tRNA-loading factor in unconventional translation initiation events, such as non-AUG translation initiation, and an alternative factor of eIF2 when the active eIF2 is limited.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant fragment of human eIF-2A (448-576 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody recognizes the protein translated from the gene EIF2A not to be confused with the protein translated from the gene EIF2S1, often referred to as eIF2-alpha in literature, and with which it shares the common name EIF2A.
- RRID
-
AB_2629818 (BioLegend Cat. No. 690302)
Antigen Details
- Structure
- 585 amino acids with a predicted molecular weight of 65kD. Contains two to four WD-repeats at the N-terminus and a coiled-coil at the C-terminus.
- Distribution
-
Cytoplasm and nucleus.
- Function
- Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits.
- Interaction
- EIF2AK2, EIF3A, RIOK2, STAT1.
- Biology Area
- Cell Biology, Protein Synthesis
- Antigen References
-
1. Zoll WL, et al. 2002. J. Biol. Chem. 277:37079.
2. Kashiwagi K, et al. 2014. J. Struct. Funct. Genomics. 15:125.
3. Adams SL, et al. 1975. J. Biol. Chem. 250:9083.
4. Starck SR, et al. 2012. Science. 336:1719.
5. Starck SR, et al. 2008. PLoS One. 3:e3460.
6. Ingolia NT, et al. 2009. Science. 324:218.
7. Ingolia NT, et al. 2011. Cell. 147:789.
8. Kim JH, et al. 2011. EMBO J. 30:2454.
9. Ventoso I, et al. 2006. Genes Dev. 20:87. - Gene ID
- 83939 View all products for this Gene ID
- UniProt
- View information about elF-2A on UniProt.org
Related FAQs
Other Formats
View All elF-2A Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-eIF-2A | P83A7A8 | WB |
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