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Purified anti-EPO Antibody

Pricing & Availability
Clone
M0312E3 (See other available formats)
Regulatory Status
RUO
Other Names
Erythropoietin, MVCD2, EP, Epoetin
Isotype
Mouse IgG2b, κ
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Product Citations
publications
M0312E3_EPO_Purified_Antibody_IP_091714
Immunoprecipitation/Western blot analysis using EPO antibody (M0312E3). 100 ng of recombinant human EPO (Cat No. 587106) was tested in 1 ml of immunoprecipitation lysis buffer. Lane 1 was immunoprecipitated with mouse IgG control antibody and lane 2 was immunoprecipitated with EPO antibody (M0312E3). Western blot analysis was performed using goat polyclonal anti-EPO antibody generated in house.
  • M0312E3_EPO_Purified_Antibody_IP_091714
    Immunoprecipitation/Western blot analysis using EPO antibody (M0312E3). 100 ng of recombinant human EPO (Cat No. 587106) was tested in 1 ml of immunoprecipitation lysis buffer. Lane 1 was immunoprecipitated with mouse IgG control antibody and lane 2 was immunoprecipitated with EPO antibody (M0312E3). Western blot analysis was performed using goat polyclonal anti-EPO antibody generated in house.
Cat # Size Price Save
662202 100 µg ¥58,300
Description

EPO is the major regulator of differentiation, proliferation, and survival of erythroid progenitors. Hypoxia induces erythropoiesis which causes cells to produce hypoxia inducible factor (HIF) that is directly involved in EPO expression. HIF-Prolyl hydroxylase (HIF-PH) is a sensor of levels of iron, oxygen, and metabolic activity. High levels of HIF protein induce EPO production in the kidneys and liver, and mobilize iron to support erythropoiesis. EPO has been used in the treatment of anemia associated with a number of conditions, such as chronic kidney disease, cancer patients on chemotherapy, and antiviral HIV therapy.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human EPO
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IP - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunoprecipitation. This antibody can be used at 1 - 5 µg/mL for immunoprecipitation. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564245 (BioLegend Cat. No. 662202)

Antigen Details

Structure
165 amino acid protein with a predicted molecular weight of approximately 18 kD. It migrates at approximately 37-57 kD as a result of its glycosylation.
Distribution
EPO is primarily expressed in kidney (70-90% of the total EPO) and liver cells. EPO is expressed by hepatocytes, cortical interstitial fibroblasts in ischemic kidneys, astrocytes, and neurons under hypoxic conditions.
Function
EPO is the major regulator of differentiation, proliferation, and survival of erythroid progenitors. EPO expression is induced by hypoxia, HIF, and other transcription factors. EPO also plays a role in neuroprotection.
Interaction
Erythroid progenitor cells
Ligand/Receptor
EpoR (homodimer)
Cell Type
Fibroblasts, Neurons
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Shoemaker CB, et al. 1986. Mol. Cell. Biol. 6:849.
2. McDonald JD, et al. 1986. Mol. Cell. Biol. 6:842.
3. Chin K, et al. 2000. Brain Res. Mol. Brain Res. 81:29.
4. Snow JW, et al. 2009. J. Biol. Chem. 284:29310.
5. Kassouf MT, et al. 2010. Genome Res. 2010:20:1064.
6. Paliege A, et al. 2010. Kidney Int. 77:312.
7. Sinclair AM, et al. 2010. Blood 115:4264.
8. Alnaeeli M, et al. 2012. Anat. Res. Int. 2012:953264.

Gene ID
2056 View all products for this Gene ID
UniProt
View information about EPO on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/24/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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