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- Clone
- W16108A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Flotillin 1, Flot-1, Integral Membrane Component of Caveolae
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot of anti-Flotillin-1 antibody (clone W16108A) and isotype-matched IgG2a control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Flotillin-1 antibody, or rat IgG2a, κ isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-rat secondary antibody. Enhanced chemiluminescence was used as the detection system. M: Molecular weight marker; brain lysates: 20µg; Myc-tagged Flotillin-1 or GST-tagged Flotillin-2 recombinant protein: 20 ng. -
ICC staining of anti-Flotillin-1 antibody (clone W16108A) on Hela cells. The cells were transfected with plasmids carrying CRISPR gRNA against Flotillin-1, Cas9 nuclease, and GFP. Two days after transfection the cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI. Arrowheads: transfected cells. Images were captured with a 60X objective. Scale bar: 25 µm.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 849801 | 25 µg | ¥28,380 | |
| 849802 | 100 µg | ¥71,060 |
The flotillin family is composed of two highly homologous proteins, flotillin-1 and flotillin-2 that share ~50% amino acid sequence similarity. Flotillins are ubiquitously expressed, membrane-associated proteins, and were shown to be associated with lipid microdomains called membrane rafts. Flotillins are present in various cellular compartments, including the plasma membrane and endosomes, and they have been suggested to function in cellular signaling and membrane trafficking processes, including endocytosis and endosomal trafficking.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human Flotillin-1 recombinant protein corresponding to amino acids 1-270 expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunocytochemistry microscopy, a concentration range of 1.0-5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody specifically recognizes Flotillin-1 but not Flotillin-2, verified using WB.
- Product Citations
-
- RRID
-
AB_2687309 (BioLegend Cat. No. 849801)
AB_2687309 (BioLegend Cat. No. 849802)
Antigen Details
- Structure
- Flotillin-1 is a 427 amino acid protein with a molecular mass of 47 kD.
- Distribution
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Tissue distribution: Flotillin-1 is ubiquitously expressed. </br>Cellular distribution: Flotillin-1 is mainly expressed in the cytosol and endosomes.
- Function
- Flotillins are thought to interact with the cytoskeleton and function in a number of cellular processes such as endocytosis.
- Interaction
- Flotillin-1 interacts with cbl-associated protein/ponsin (CAP).
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
- Antigen References
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1. Otto G et al., 2011. Journal of Cell Science. 124: 3933.
- Gene ID
- 10211 View all products for this Gene ID
- UniProt
- View information about Flotillin-1 on UniProt.org
Other Formats
View All Flotillin-1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Flotillin-1 | W16108A | WB,ICC |
| HRP anti-Flotillin-1 | W16108A | WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Flotillin-1
Western blot of anti-Flotillin-1 antibody (clone W16108A) an... 
ICC staining of anti-Flotillin-1 antibody (clone W16108A) on... -
HRP anti-Flotillin-1
Western blot of HRP anti-Flotillin-1 antibody (clone W16108A...
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