- Clone
- 2S7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GEMIN 2, SIP1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![2S7_Purified_Gemin-2_Antibody_041019 2S7_Purified_Gemin-2_Antibody_041019](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2S7_Purified_Gemin-2_Antibody_041019.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-Gemin 2 antibody (clone 2S7) and isotype-matched IgG1, κ control. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of HeLa cell lysate. The blots were incubated with 2 µg/mL of clone 2S7 or mouse IgG1, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # | Size | Price | Save |
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862901 | 25 µg | ¥23,320 | |
862902 | 100 µg | ¥60,720 |
Gemin 2 is a component of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. The SMN complex localizes to the cytosol and nucleus of eukaryotic cells. Gemin 2 is the only gemin protein that has no homology to any other gemins. Recent studies have suggested that the role of Gemin 2 is to organize 5 Sm proteins into a partial ring for snRNA binding.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
ICC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Battle D, et al. 2007. J Biol Chem. 282(38):27953 (WB, ICC)
- Product Citations
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- RRID
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AB_2810755 (BioLegend Cat. No. 862901)
AB_2810756 (BioLegend Cat. No. 862902)
Antigen Details
- Structure
- Gemin 2 is a 280 amino acid protein with an apparent molecular mass of ~ 32 kD.
- Distribution
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Tissue Distribution: Brain, skeletal muscle, gallbladder, and testis
Cellular Distribution: Nuclear and cytosol - Function
- Gemin 2 is a component of the SMN complex that is essential for the formation of snRNP complexes. It organizes 5 Sm proteins into a partial ring for snRNA binding.
- Interaction
- Gemin 2 binds to SM proteins and is found in complex with other SMN/GEMIN proteins.
- Cell Type
- Neurons
- Biology Area
- Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroscience, Synaptic Biology, Transcription Factors
- Antigen References
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- Battle D, et al. 2007. J Biol Chem. 282(38):27953
- Gupta K, et al. 2015. J Biol Chem. 290(33):20185
- Gene ID
- 8487 View all products for this Gene ID 66603 View all products for this Gene ID 84404 View all products for this Gene ID
- UniProt
- View information about Gemin 2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Gemin 2 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-Gemin 2 | 2S7 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Gemin 2
Western blot of purified anti-Gemin 2 antibody (clone 2S7) a...
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