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Purified anti-GRB2 Antibody

Pricing & Availability
Clone
5G3-E7-F11 (See other available formats)
Regulatory Status
RUO
Other Names
Growth Factor Receptor Bound Protein 2, ASH
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5G3-E7-F11_Purified_GRB2_Antibody_1_061819.png
Western blot of purified anti-GRB2 antibody (clone 5G3-E7-F11). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5G3-E7-F11_Purified_GRB2_Antibody_1_061819.png
    Western blot of purified anti-GRB2 antibody (clone 5G3-E7-F11). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5G3-E7-F11_Purified_GRB2_Antibody_2_061819.png
    IHC staining of purified anti-GRB2 antibody (clone 5G3-E7-F11) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R,, the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 5G3-E7-F11_Purified_GRB2_Antibody_3_061819.png
    IHC staining of purified anti-GRB2 antibody (clone 5G3-E7-F11) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 5G3-E7-F11_Purified_GRB2_Antibody_4_061819.png
    IHC staining of purified anti-GRB2 antibody (clone 5G3-E7-F11) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Save
867101 25 µg ¥23,320
867102 100 µg ¥60,720
Description

Growth factor receptor bound protein 2 (GRB2) is an adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. GRB2 is ubiquitously expressed, and has been shown to be essential to various cellular functions. Due to its involvement in cell growth, cell proliferation and metabolism, GRB2 has been linked to multiple tumor malignancies, and thus is a potential therapeutic target for cancer therapy.  Recent neurodevelopmental studies also suggest that variations in intracellular levels of adapter proteins such as GRB2 may represent an underlying mechanism that links exogenous signals to cell fate determination.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human GRB2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 2 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810797 (BioLegend Cat. No. 867101)
AB_2810797 (BioLegend Cat. No. 867102)

Antigen Details

Structure
GRB2 is a 217 amino acid protein with predicted and observed molecular mass of ~25 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, nucleus, cytosol, endosome, and Golgi apparatus

Function
GRB2 is an adapter protein that binds to activated SH2-containing proteins and plays an important role in determining cell fate.
Interaction
EGF receptors, PDGF receptors, and other proteins containing SH2 or SH3 domains.
Cell Type
Microglia, Neurons
Biology Area
Cancer Biomarkers, Cell Death, Neurodegeneration, Neuroscience, Signal Transduction
Molecular Family
Adaptor Proteins
Antigen References
  1. Ijaz M, et al. 2018. Protein Pept Lett. 24(12):1084
  2. Paff M, et al. 2014. Hum Vaccin Immunother. 10(11):3322
  3. Belov AA and Mohammadi M. 2012. Sci Signal. 5(249):pe49
Gene ID
2885 View all products for this Gene ID 14784 View all products for this Gene ID 81504 View all products for this Gene ID
UniProt
View information about GRB2 on UniProt.org
Go To Top Version: 1    Revision Date: 06/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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