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Purified anti-Haptoglobin Antibody

Pricing & Availability
Clone
BL28630 (See other available formats)
Regulatory Status
RUO
Other Names
Zonulin, Alpha Polypeptide, Beta polypeptide, Binding Peptide, HP2ALPHA2, HPA1S, BP, Haptoglobin Alpha(1S)-Beta, Haptoglobin Alpha(2FS)-Beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BL28630_PURE_Haptoglobin_Antibody_070319.png
Western blot of purified anti-Haptoglobin antibody (clone BL28630). Lane 1: Molecular weight marker; Lane 2: 1 µg of human plasma; Lane 3: 2 µg of human plasma; Lane 4: 5 µg of human plasma; Lane 5: 10 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28630_PURE_Haptoglobin_Antibody_070319.png
    Western blot of purified anti-Haptoglobin antibody (clone BL28630). Lane 1: Molecular weight marker; Lane 2: 1 µg of human plasma; Lane 3: 2 µg of human plasma; Lane 4: 5 µg of human plasma; Lane 5: 10 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Save
863001 25 µg ¥23,320
Description

Haptoglobin is a protein that is mainly expressed by hepatic cells in the liver. Haptoglobin is an acute-phase antioxidant that binds to free hemoglobin (Hb) in the plasma and inhibits the oxidative activity of hemoglobin. Individuals carrying the haptoglobin 2-2 genotype have decreased affinity to Hb and are at a higher risk of developing cerebral aneurysm-related complications due to impaired anti-inflammatory pathways. This highlights haptoglobin as part of the Hb scavenging mechanism, which may be critical for clearing Hb after intracranial bleeding and neuroprotection.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Haptoglobin corresponding to amino acids 168 – 391 expressed by E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814603 (BioLegend Cat. No. 863001)

Antigen Details

Structure
Haptoglobin is a 406 amino acid protein with predicted and observed molecular mass of ~45 kD.
Distribution

Tissue Distribution: Liver and blood plasma
Cellular Distribution: Extracellular and cytosol

Function
Haptoglobin is a part of the hemoglobin scavenging mechanism that clears Hb released into the central nervous system after intracranial bleeding.
Interaction
Haptoglobin forms tetramers of two alpha and two beta chains. It binds to hemoglobin and CD163.
Biology Area
Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Blood Group Markers
Antigen References
  1. Bulters D, et al. 2018. Nat Rev Neurol. 14(7):416.
  2. Blackburn SL, et al. 2018. Front Physiol. 9:592.
  3. Bamm VV, et al. 2017. Metab Brain Dis. 32(1):19-34.
Gene ID
3240 View all products for this Gene ID
UniProt
View information about Haptoglobin on UniProt.org
Go To Top Version: 1    Revision Date: 07/03/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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