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Purified anti-HOXB4 Antibody

Pricing & Availability
Clone
W22219B (See other available formats)
Regulatory Status
RUO
Other Names
Homeobox protein Hox-B4, Homeobox protein Hox-2.6, Homeobox protein Hox-2F, Homeobox B4, HOX2F
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W22219B_PURE_HOXB4_WB_100724
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-HOXB4 (clone W22219B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22219B_PURE_HOXB4_WB_100724
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-HOXB4 (clone W22219B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Save
661451 25 µg ¥37,400
661452 100 µg ¥94,600
Description

HOXB4 is a member of the Homeobox (HOX) gene family, which plays a role in regulating embryonic development and maintaining adult tissue homeostasis. HOXB4 is a sequence-specific transcription factor with a homeobox DNA-binding domain. In hematopoiesis, HOXB4 enhances the self-renewal capacity of hematopoietic stem cells (HSCs), which are responsible for the continuous production of blood cells. Overexpression of HOXB4 in HSCs has been shown to lead to an expansion of these stem cells without inducing leukemogenesis. By binding to specific DNA sequences, HOXB4 activates a network of target genes that promote stem cell proliferation and inhibit differentiation, thus maintaining the stem cell pool.

HOXB4 also plays a pivotal role in organogenesis, particularly in the development of the spinal cord and the patterning of the vertebral column. During embryonic development, HOXB4 is expressed in a specific spatial and temporal manner, contributing to the formation of structures along the anterior-posterior axis. Its expression helps determine the identity and positional information of various segments, ensuring that body parts develop in the correct locations. In vertebrates, this function is crucial for the proper segmentation and alignment of the spine, as well as for the formation of the nervous system. Furthermore, aberrations in HOXB4 expression or function have been associated with various pathological conditions, including cancers. Dysregulated HOXB4 expression can lead to the uncontrolled proliferation of cells, contributing to tumorigenesis. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human HOXB4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is not suitable for use in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P) or immunocytochemistry (ICC).

This antibody does not cross react with mouse HOXB4 in Western blotting (WB).

Antigen Details

Structure
Contains a homeobox DNA-binding domain
Distribution

Expressed in embryonic tissue and hematopoietic stem cells/localized in nucleus

Function
A transcription factor that regulates hematopoietic stem cell self-renewal or development of anterior-posterior axis
Interaction
Binds to homeobox DNA sequence
Biology Area
Neuroscience, Stem Cells, Transcription Factors
Antigen References
  1. Gould A, et al. 1998. Neuron. 21:39-51.
  2. Antonchuk J, et al. 2002. Cell. 109:39-45.
  3. Kyba M, et al. 2002. Cell. 109:29-37.
  4. Bowles KM, et al. 2006. Stem Cells. 24:1359-69.
Gene ID
3214 View all products for this Gene ID
UniProt
View information about HOXB4 on UniProt.org
Go To Top Version: 1    Revision Date: 10/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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