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- Clone
- 4B4 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- Heat shock factor protein 1, heat shock transcription factor 1, HSF 1, HSTF 1
- Previously
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Covance Catalog# MRT-5113
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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IHC staining of purified anti-HSF1 antibody (clone 4B4) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. Following a biotinylated goat anti-rat linking reagent, BioLegend’s Ultra-Streptavidin (USA) HRP labeling reagent and DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective
HSF1 is a DNA-binding protein that exists as an inactive monomer in a complex with Hsp40/Hsp70 and Hsp90. Upon stress, HSF1 is released from the chaperone complex and trimerizes. HSF1 is then transported into the nucleus where it is hyperphosphorylated and binds to DNA containing heat shock elements (HSE) and activates transcription of proteins such as HSP70. HSP70 is known to inhibit the tau and alpha synuclein aggregation seen in Alzheimer’s Disease and Parkinson’s Disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- This monoclonal antibody was raised against mouse recombinant HSF-1 protein and was mapped to amino acids 425-439.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
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IHC-P - Quality tested
WB, ICC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Mathew A, et al. 2001. Mol. Cell. Biol. 21:7163. (WB, ICC)
- RRID
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AB_2564892 (BioLegend Cat. No. 825801)
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Gene ID
- 3297 View all products for this Gene ID
- UniProt
- View information about HSF1 on UniProt.org
Related Pages & Pathways
Pathways
Other Formats
View All HSF1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-HSF1 | 4B4 | IHC-P,WB,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-HSF1
IHC staining of purified anti-HSF1 antibody (clone 4B4) on f...
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