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Purified anti-human ATG3 Antibody

Pricing & Availability
Clone
A19237B (See other available formats)
Regulatory Status
RUO
Other Names
Autophagy Related 3
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A19237B_PURE_ATG3_Antibody_052322
Human paraffin-embedded testis tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ATG3 (clone A19237B) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A19237B_PURE_ATG3_Antibody_052322
    Human paraffin-embedded testis tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ATG3 (clone A19237B) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # Size Price Save
379702 100 µg ¥63,360
Description

Human autophagy related 3, ATG3, is a 314 amino acid protein with a molecular mass of 36 kD, which is a ubiquitin-like modifier similar to the canonical E2 enzyme. ATG3 is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy is the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway which plays an important role in controlling normal growth but may be defective in tumor cells. ATG3 is essential for autophagocytosis, which is a starvation-induced process responsible for transport of cytoplasmic proteins to the lysosome/vacuole. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922609 (BioLegend Cat. No. 379702)

Antigen Details

Structure
Human ATG3 is a 314 amino acid protein with a molecular mass of 36 kD
Distribution

Expressed in testis and bone marrow stromal cell

Function
E2-conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2, or MAP1LC3A).
Interaction
Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
Ligand/Receptor
ATG8
Biology Area
Cell Biology, Cell Death, Immunology
Molecular Family
Apolipoproteins, Autophagosome Markers
Antigen References
  1. Isei T, et al. 2002. J Biol Chem. 277:13739.
  2. Silke M. et al. 2009. Hum Genet. 128:453.
  3. Ozlem O, et al. 2012. Apoptosis. 17:810.
Gene ID
64422 View all products for this Gene ID
UniProt
View information about ATG3 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/23/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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