Purified anti-human B7-H4 Antibody anti-B7-H4

Clone: MIH43 (See other available formats)
Regulatory Status: RUO
Other Names: VTCN1, B7h.5
Isotype: Mouse IgG1, κ
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MIH43_Purified_B7-H4_Antibody_FC_042613 — Human B7-H4 transfected P815 cells were stained with purified B7-H4 (clone MIH43, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.

Cat #	Size	Price	Save
358101	25 µg	¥22,220
358102	100 µg	¥58,300

Description

B7-H4, also known as VTCN1, is a type I transmembrane protein and member of the B7 family. Its extracellular region consists of one IgV-like and one IgC-like domain. B7-H4 expression has been reported on activated T cells, B cells, monocytes, and dendritic cells. On T cells, B7-H4 inhibits proliferation, cytokine secretion, and cytotoxicity. B7-H4 is also expressed by different carcinomas including renal, gastric, breast, ovarian and melanoma. Its expression is associated with a poor prognosis.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) includes immunofluorescence².
RRID: AB_2562078 (BioLegend Cat. No. 358101)
AB_2562078 (BioLegend Cat. No. 358102)

Antigen Details

Structure: Type I transmembrane protein, member of the B7 family, one IgV-like, one IgC-like domain
Distribution: Activated T cells, B cells, monocytes, and dendritic cells; different cancer types including melanoma, renal, gastric, breast and ovarian
Function: Inhibits T cell proliferation, cytokine secretion, and development of cytotoxicity
Cell Type: B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Antigen References: 1. Fauci JM, et al. 2012. Gynecol. Oncol. 127:420.
2. Chen C, et al. 2012. J. Immunother. 35:354.
3. Guo G, et al. 2012. Clin. Rheumatol. 31:271.
4. Arigami T, et al. 2010. J. Surg. Oncol. 102:748.
Gene ID: 79679 View all products for this Gene ID
UniProt: View information about B7-H4 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All B7-H4 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human B7-H4	MIH43	FC,IHC-F,ICC
PE anti-human B7-H4	MIH43	FC
PE/Cyanine7 anti-human B7-H4	MIH43	FC
APC anti-human B7-H4	MIH43	FC
PerCP/Cyanine5.5 anti-human B7-H4	MIH43	FC
PE/Dazzle™ 594 anti-human B7-H4	MIH43	FC
TotalSeq™-A0395 anti-human B7-H4	MIH43	PG
TotalSeq™-C0395 anti-human B7-H4	MIH43	PG
APC/Fire™ 750 anti-human B7-H4	MIH43	FC
TotalSeq™-B0395 anti-human B7-H4	MIH43	PG

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 05/05/2015

For Research Use Only. Not for diagnostic or therapeutic use.

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