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- Clone
- S20015E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL5RA, IL5R, CDw125, HSIL5R3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood granulocytes were stained with anti-human CD193 (CCR3) APC and purified anti-human CD125 (IL-5Rα) (clone S20015E) (left) or purified mouse IgG1, κ isotype control (right), followed by PE anti-mouse IgG.
CD125, also known as IL5 receptor α-chain, is a 60 kD type I transmembrane protein that binds IL-5 and associates in a heterodimer with the signal-transducing β subunit CD131 to form the IL-5 receptor. The affinity of CD125 for IL-5 is increased when is associated with CD131. The cytoplasmic region of CD125 activates the JAK2/STAT5 pathway and the kinase Btk. CD125 is expressed by eosinophils, basophils, mast cells, B1 cells and a subset of resting B2 cells and have a central role in the differentiation, activation and survival of these cells. Due to the restricted expression of this molecule, it is considered a potential therapeutic target for allergic inflammatory conditions.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse IL-5Rα transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2927968 (BioLegend Cat. No. 384702)
Antigen Details
- Structure
- Type I transmembrane protein, 60 kD
- Distribution
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Eosinophils, basophils, mast cells, B1 cells, subset of resting B2 cells
- Function
- Role in eosinophils and basophils differentiation, activation and survival
- Interaction
- JAK2/STAT, Btk, SOX4
- Ligand/Receptor
- IL-5
- Cell Type
- B cells, Basophils, Eosinophils
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Buchheit KM, et al. 2020. J Allergy Clin Immunol. 145:1574-1584.
- Lin X, et al. 2020. Inflammation. 43:1797-1805.
- Gorski SA, et al. 2019. PLoS One. 14:e0221113.
- Varricchi G, et al. 2016. Curr Opin Allergy Clin Immunol.16:186-200.
- Beekman JM. et al. 2009. Blood. 114:3917-27.
- Lei JT. et al. 2008. J Leukoc Biol. 84:499-509.
- Gene ID
- 3568 View all products for this Gene ID
- UniProt
- View information about CD125 on UniProt.org
Related FAQs
Other Formats
View All CD125 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD125 (IL-5Rα) | S20015E | FC |
| PE anti-human CD125 (IL-5Rα) | S20015E | FC |
| TotalSeq™-C1287 anti-human CD125 (IL-5Rα) | S20015E | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD125 (IL-5Rα)
Human peripheral blood granulocytes were stained with anti-h... -
PE anti-human CD125 (IL-5Rα)
Human peripheral blood granulocytes were stained with anti-h... -
TotalSeq™-C1287 anti-human CD125 (IL-5Rα)
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