Purified anti-human CD133 Antibody, CD133, W6B3C1

Clone: W6B3C1 (See other available formats)
Regulatory Status: RUO
Other Names: Prominin-1, PROM1, AC133
Isotype: Mouse IgG1, κ
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NCCIT cells were stained with purified anti-human CD133 (clone W3B3C1) (filled histogram) or Mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.

Cat #	Size	Price	Save
397902	100 µg	¥60,060

Description

CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: WERI-RB-1 human retinoblastoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: W6B3C1 antibody does not compete against AC133, 293C2, clone 7, S16015F, S16016B, or S16016E antibody binding to target.
RRID: AB_2820062 (BioLegend Cat. No. 397902)

Antigen Details

Structure: 120 kD pentaspan transmembrance glycoprotein with 5 transmembrane domains.
Distribution: Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells.
Function: May play a role in cell differentiation, proliferation, and apoptosis.
Interaction: NAT8; NAT8B; CDHR1
Cell Type: Hematopoietic stem and progenitors, Leukemia, Leukocytes
Biology Area: Cancer Biomarkers, Cell Biology, Immuno-Oncology
Molecular Family: CD Molecules
Antigen References: Yin AH, et al. 1997. Blood. 90:5002.

Miraglia S, et al. 1997. Blood. 90:5013.

Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.
Gene ID: 8842 View all products for this Gene ID
UniProt: View information about CD133 on UniProt.org

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Other Formats

View All CD133 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD133	W6B3C1	FC
PE anti-human CD133	W6B3C1	FC
APC anti-human CD133	W6B3C1	FC
FITC anti-human CD133	W6B3C1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10/02/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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