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- Clone
- S22019F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD158H, Killer Cell Immunoglobulin-Like Receptor 2DS1, KIR2DS1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes were stained with purified anti-human CD158h (KIR2DS1) (clone S22019F) (left) or purified mouse IgG1, κ isotype control (right), followed by goat anti-mouse IgG PE. Cells were costained with anti-human CD56 (clone QA17A16) APC. -
Human peripheral blood mononuclear cells were stained with purified anti-human CD158h (KIR2DS1) (clone S22019F) followed by goat anti-mouse IgG APC and anti-human CD158a (KIR2DL1) (clone HP-DM1) PE. Cells were costained with anti-human CD56 (clone QA17A16) Pacific Blue™. Data shown was gated on the CD56+ lymphocyte population.
CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIMs and have activation functions. Fifteen polymorphic KIR genes have been reported in humans. CD158h is a 50 kD, single-pass glycoprotein with two extracellular Ig domains that contains a short cytoplasmic tail and does not contain an intracellular ITIM domain. It is expressed primarily on a subset of NK cells and a subset of T cells. KIR2DS1 is reported to bind certain HLA-C antigens on target cells (HLA-C*02, HLA-C*04, HLAC*06, for example).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KIR2DS1 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Based on in-house testing, clone S22019F does not react to other KIR protein family members.
Antigen Details
- Structure
- 50 kD two Ig-like extracellular domain glycoprotein
- Distribution
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Subset of NK cells, small subset of T Cells
- Function
- NK cell regulation
- Ligand/Receptor
- Some HLA-C molecules
- Cell Type
- Lymphocytes, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
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- Biassoni, R. et al. 1996. J Exp Med. 183:645-50.
- Stewart CA, et al. 2005. Proc Natl Acad Sci. 102:13224-9.
- Lanier, LL. 2005. Annu Rev Immunol. 23:225-74.
- Gene ID
- 3806 View all products for this Gene ID
- UniProt
- View information about CD158h on UniProt.org
Other Formats
View All CD158h Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD158h (KIR2DS1) | S22019F | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD158h (KIR2DS1)
Human peripheral blood lymphocytes were stained with purifie... 
Human peripheral blood mononuclear cells were stained with p...
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