United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Clone
- S22012A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NKAT8, Killer Cell Immunoglobulin-Like Receptor 2DS4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with anti-human CD56 (clone QA17A16) APC and purified anti-human CD158i (KIR2DS4) (clone S22012A) (left) or purified mouse IgG1, κ isotype control (right), followed by goat anti-mouse IgG PE.
CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Expression is highly polymorphic between individuals and is expressed generally on a subset of NK cells and a subset of T cells. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. CD158i is a two extracellular Ig domain glycoprotein that displays a short cytoplasmic tail. KIR2DS4 is reported to bind to certain HLA-C molecules and HLA-A*11.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KIR2DS4-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Based on in-house testing on different KIR-expressing transfectants, clone S22012A does not interact with other KIR protein family members.
- RRID
-
AB_3662395 (BioLegend Cat. No. 335104)
Antigen Details
- Structure
- Two Ig-like extracellular domain glycoprotein
- Distribution
-
Subset of NK cells, subset of T Cells
- Function
- NK cell regulation
- Ligand/Receptor
- HLA-C, HLA-A*11
- Cell Type
- Lymphocytes, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
- Bottino C, et al. 1996. Eur J immunol. 26:1816-24.
- Graef T, et al. 2009. J Exp Med. 206:2557-72.
- Campbell KS, et al. 1998. Eur J Immunol. 28:599-609.
- Gene ID
- 3809 View all products for this Gene ID
- UniProt
- View information about CD158i on UniProt.org
Related FAQs
Other Formats
View All CD158i Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD158i (KIR2DS4) | S22012A | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD158i (KIR2DS4)
Human peripheral blood lymphocytes were stained with anti-hu...
Follow Us