- Clone
- 1H7/CD221 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Insulin-like growth factor 1 receptor (IGF1R)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1H7slashCD221_Pure_042211 1H7slashCD221_Pure_042211](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1H7slashCD221_Pure_042211.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with purified CD221 (clone 1H7/CD221) (filled histogram) or mouse IgG1 isotype control (open histogram), followed by biotinylated anti-mouse IgG and Sav-PE.
CD221, also known as insulin-like growth factor 1 receptor (IGF1R), is a 135 kD transmembrane receptor. It is widely expressed on lymphoid and myeloid cells as well as non-haematopoietic cells. CD221 is a tyrosine kinase receptor and the ligands for CD221 are insulin-like growth factor 1 and 2 (IGF-1 and IGF-2), the polypeptide hormones similar in molecular structure to insulin. IGF-1/2 binding activates downstream PI3K and MAPK pathways and plays an important role in cell growth. Recent studies reveal its function of recruiting immune cells during tissue repair.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- IGF-1 receptor purified from human placentas
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IHC, IP, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone has been published as 1H7. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining of frozen tissue sections2, and blocking functions2-5.
This product is offered for sale and sold pursuant to a license to seller by City of Hope and may be used only as a research reagent. Use for any other purpose is expressly not authorized. -
Application References
(PubMed link indicates BioLegend citation) -
- Li SL, et al. 1993. Biochem. Bioph. Res. Co. 196:92. (WB IP)
- Li SL, et al. 2000. Cancer Immunol. Immun. 49:243. (IHC Block)
- Ye JJ, et al. 2003. Horm. Metab. Res. 35:836. (Block)
- Kusada Y, et al. 2008. J. Biochem. 143:9. (Block)
- Ohtani M, et al. 2009. Biosci. Trends. 3:131. (Block)
- RRID
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AB_10896950 (BioLegend Cat. No. 351802)
Antigen Details
- Structure
- IGF-1R contains 2 α subunits and 2 β subunits, 135 kD
- Distribution
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Haematopoietic cells and non-haematopoietic cells
- Function
- Cell growth and immune cells recruitment
- Ligand/Receptor
- IGF-1, IGF-2
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Baudler S, et al. 2005. J. Immunol. 174:5516.
2. Sharp LL, et al. 2005. Nat. Immunol. 6:73. - Gene ID
- 3480 View all products for this Gene ID
- UniProt
- View information about CD221 on UniProt.org
Related FAQs
Other Formats
View All CD221 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD221 (IGF-1R) | 1H7/CD221 | FC,IHC,IP,WB |
PE anti-human CD221 (IGF-1R) | 1H7/CD221 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD221 (IGF-1R)
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD221 (IGF-1R)
Human peripheral blood lymphocytes were stained with CD3 APC...
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