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- Clone
- W21097F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DNAM-1, PTA1, TLiSA1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and purified anti-human CD226 (DNAM-1) (clone W21097F) (left), or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG PE.
DNAM-1 (CD226) is a ~65 kD glycoprotein expressed on cell surface of T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells and a member of the immunoglobulin (Ig)-superfamily containing 2 Ig-like domains of the V-set. The ligands for CD226 are the poliovirus receptor (CD155) and its family member nectin-2 (CD112), which are broadly expressed on epithelial, endothelial and neuronal cells. CD226 is physically associated with LFA-1 in NK cells and activated T cells, and involved in LFA-1-mediated signaling.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human DNAM-1 Transfected Cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3083199 (BioLegend Cat. No. 387802)
Antigen Details
- Structure
- Ig domain containing adhesion molecule
- Distribution
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Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells
- Function
- Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell
- Interaction
- Interacts with LFA-1
- Ligand/Receptor
- PVR (CD155) and Nectin-2 (CD112)
- Cell Type
- B cells, Macrophages, Megakaryocytes, Monocytes, NK cells, Platelets, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Shibuya A, et al. 1996. Immunity. 4:573-81.
- Tahara-Hanaoka S, et al. 2004. Int Immunol. 16:533-8.
- Pende D, et al. 2005. Mol. Immunol. 42:463-9.
- Gene ID
- 10666 View all products for this Gene ID
- UniProt
- View information about CD226 on UniProt.org
Other Formats
View All CD226 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD226 (DNAM-1) | W21097F | FC |
| Purified anti-human CD226 (DNAM-1) | W21097F | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD226 (DNAM-1)
Human peripheral blood lymphocytes were stained with anti-hu... -
Purified anti-human CD226 (DNAM-1)
Human peripheral blood lymphocytes were stained with anti-hu...
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