Purified anti-human CD244 2B4 Antibody anti-CD244

Clone: 2-69 (See other available formats)
Regulatory Status: RUO
Other Names: 2B4, NAIL, SLAMF4
Isotype: Mouse IgG2a, κ
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Product Citations: publications

2-69_PURE_CD244_Antibody_030818 — Human lysed whole blood stained with CD56 APC and purified CD244 (clone 2-69, top) or mouse IgG2a, κ isotype control (bottom), followed by anti-mouse IgG2a FITC.

Cat #	Size	Price	Save
393502	100 µg	¥27,060

Description

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8⁺ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human NK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IP, Cyt - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: redirected killing assays (rADCC) in NK cells¹.
RRID: AB_2728427 (BioLegend Cat. No. 393502)

Antigen Details

Structure: 38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution: NK, T subset, monocytes, basophils, and eosinophils
Function: Activation and inhibition of NK cell function
Ligand/Receptor: CD48
Cell Type: Basophils, Eosinophils, Monocytes, NK cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Lee KM, et al. 2003. J. Immunol. 170:4881.

Chlewicki LK, et al. 2008. J. Immunol. 180:8159.

Messmer B, et al. 2006. J. Immunol. 176:4646.

Boles KS, et al. 2001. Immunol. Rev. 181:234.
Gene ID: 51744 View all products for this Gene ID
UniProt: View information about CD244 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD244 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD244 (2B4)	2-69	FC,Cyt,IP
PE anti-human CD244 (2B4)	2-69	FC
PE/Dazzle™ 594 anti-human CD244 (2B4)	2-69	FC
Brilliant Violet 421™ anti-human CD244 (2B4)	2-69	FC
FITC anti-human CD244 (2B4)	2-69	FC
APC anti-human CD244 (2B4)	2-69	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/12/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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