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- Clone
- QA18A45 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Low affinity IL-2R, IL-2R α chain, Tac, p55
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
PHA stimulated (3days) human peripheral blood lymphocytes were stained with purified anti-human CD25 recombinant (clone QA18A45) (left) or purified mouse IgG1a, κ isotype control (right), followed by goat anti-mouse IgG PE. Cells were costained with CD3 APC.
CD25 is a 55 kD type I transmembrane glycoprotein also known as IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
QA18A45 clone of antibody is able to completely block the binding of clone BC96 to IL-2Rα.
- RRID
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AB_2888839 (BioLegend Cat. No. 311702)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, 55 kD
- Distribution
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Activated T cells and B cells, monocytes/macrophages, Treg
- Function
- Binds IL-2
- Interaction
- IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- Lymphocytes, T cells, Tregs
- Biology Area
- Adaptive Immunity, Cell Proliferation and Viability, Immuno-Oncology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681. - Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Related FAQs
Other Formats
View All Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD25 Recombinant Antibody | QA18A45 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD25 Recombinant Antibody
PHA stimulated (3days) human peripheral blood lymphocytes we...
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