- Clone
- 5-109 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ADA-binding protein, DPP IV ectoenzyme, EC 3.4.14.5
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD26, also known as ADA-binding protein and dipeptidyl peptidase IV (DPPIV), is a 110 kD type II membrane protein. It is a member of the peptidase and ectoenzyme family. CD26 is expressed on the membrane of mature thymocytes, T lymphocytes (upregulated upon activation), B cells, NK cells, and macrophages. CD26 cleaves off N-terminal X-Pro and X-Ala dipeptides from polypeptides. It plays an integral role as a costimulatory molecule in T cell activation. CD26 may interact with extracellular matrix proteins such as fibronectin or collagen, CD45, and ADA.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Direct ELISA – Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. Each lot of this antibody is verified by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
- Application Notes
-
Test clone 5-109 and reference clone BA5b completely cross-block the staining on target cells.
- Additional Product Notes
-
This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone 5-109) could function as a capture antibody when paired with biotin anti-human CD26 (DDP4) (Cat No. 628153) (clone 1-10) as the detection antibody and using recombinant Human DDP4 (carrier-free) protein (Cat. Nos. 764102, 764104, and 764106) as the assay standard.
- RRID
-
AB_3662413 (BioLegend Cat. No. 335306)
Antigen Details
- Structure
- Peptidases and ectoenzyme families, type II glycoprotein, 110 kD
- Distribution
-
Thymocyte subset, memory T cells, B cells, NK cells, epithelial cells, macrophages
- Function
- Dipeptidyl peptidase, T cell costimulation, HIV entry
- Ligand/Receptor
- Adenosine-deaminase, collagen
- Cell Type
- B cells, Epithelial cells, Macrophages, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Kameoka J, et al. 1993. Science. 261:466-9.
- Dang NH, et al. 1990. J Exp Med. 172:649-52.
- Gene ID
- 1803 View all products for this Gene ID
- UniProt
- View information about CD26 on UniProt.org
Related FAQs
Other Formats
View All CD26 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD26 | 5-109 | FC,Direct ELISA |
PE anti-human CD26 | 5-109 | FC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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