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- Clone
- QA18A71 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- S152, T14, TNFRSF7
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood mononuclear cells were stained with anti-human CD3 (clone UCHT1) APC and purified anti-human CD27 recombinant (clone QA18A71) (left), or purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Data shown was gated on the lymphocyte population.
CD27, also known as S152 and T14, is a 50-55 kD type I membrane protein. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in co-stimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone QA18A71 can completely block the binding of clone O323 on target cells.
- RRID
-
AB_3662357 (BioLegend Cat. No. 321452)
Antigen Details
- Structure
- TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
- Distribution
-
Medullary thymocytes, T and B cell subsets, NK cells
- Function
- CD27 plays an important role in costimulation of T cell activation and regulation of B cell differentiation and proliferation.
- Interaction
- TRAF2, TRAF5
- Ligand/Receptor
- CD70
- Cell Type
- B cells, NK cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
- Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
- Hintzen RQ, et al. 1994. Immunol Today. 15:307-11.
- Agematsu K, et al. 1995. J Immunol. 154:3627-35.
- Gene ID
- 939 View all products for this Gene ID
- UniProt
- View information about CD27 on UniProt.org
Related FAQs
Other Formats
View All CD27 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD27 Recombinant Antibody | QA18A71 | FC |
| PE anti-human CD27 Recombinant Antibody | QA18A71 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD27 Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
PE anti-human CD27 Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a...
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