- Clone
- 24F.10C12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B7-DC, PD-L2, PDL2, B7DC
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PD-L2 cDNA
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Brown JA, et al. 2003. J. Immunol. 170:1257
- Rodig N, et al. 2003. Eur. J. Immunol. 33:3117
- Product Citations
-
- RRID
-
AB_2565428 (BioLegend Cat. No. 329613)
Antigen Details
- Structure
- B7 Immunoglobulin superfamily, 25 kD
- Distribution
-
Dendritic cells, liver, few transformed cell lines, subset of macrophages
- Function
- Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
- Ligand/Receptor
- PD-1
- Cell Type
- Dendritic cells, Macrophages
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Shin T, et al. 2003. J. Exp. Med. 198:31.
2. Liu X, et al. 2003. J. Exp. Med. 197:1721.
3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29. - Gene ID
- 80380 View all products for this Gene ID
- UniProt
- View information about CD273 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD273 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | FC,IHC-F,FA |
Biotin anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | FC |
APC anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | FC |
PE anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | FC |
Alexa Fluor® 647 anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | FC |
Purified anti-human CD273 (B7-DC, PD-L2) (Maxpar® Ready) | 24F.10C12 | FC,CyTOF® |
PE/Dazzle™ 594 anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | FC |
Brilliant Violet 421™ anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | FC |
TotalSeq™-A0008 anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | PG |
TotalSeq™-C0008 anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | PG |
Ultra-LEAF™ Purified anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | FC,IHC-F,FA |
TotalSeq™-B0008 anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | PG |
TotalSeq™-D0008 anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | PG |
KIRAVIA Blue 520™ anti-human CD273 (B7-DC, PD-L2) | 24F.10C12 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD273 (B7-DC, PD-L2)
Human monocyte-derived dendritic cells were stained with ant... -
Biotin anti-human CD273 (B7-DC, PD-L2)
Monocyte-derived dendritic cells were stained with biotin an... -
APC anti-human CD273 (B7-DC, PD-L2)
Human monocyte-derived dendritic cells were stained with CD2... -
PE anti-human CD273 (B7-DC, PD-L2)
Monocyte-derived dendritic cells stained with 24F.10C12 PE -
Alexa Fluor® 647 anti-human CD273 (B7-DC, PD-L2)
Monocyte-derived dendritic cells stained with 24F.10C12 Alex... -
Purified anti-human CD273 (B7-DC, PD-L2) (Maxpar® Ready)
Human monocyte-derived dendritic cells stained wtih 154Sm-an... -
PE/Dazzle™ 594 anti-human CD273 (B7-DC, PD-L2)
Human monocyte-derived dendritic cells were stained with ant... -
Brilliant Violet 421™ anti-human CD273 (B7-DC, PD-L2)
Human monocyte-derived dendritic cells were stained with ant... -
TotalSeq™-A0008 anti-human CD273 (B7-DC, PD-L2)
-
TotalSeq™-C0008 anti-human CD273 (B7-DC, PD-L2)
-
Ultra-LEAF™ Purified anti-human CD273 (B7-DC, PD-L2)
Human monocyte-derived dendritic cells were stained with ant... -
TotalSeq™-B0008 anti-human CD273 (B7-DC, PD-L2)
-
TotalSeq™-D0008 anti-human CD273 (B7-DC, PD-L2)
-
KIRAVIA Blue 520™ anti-human CD273 (B7-DC, PD-L2)
Human monocyte-derived dendritic cells were stained with ant...
Follow Us