Purified anti-human CD30 Antibody

Pricing & Availability
Clone
A20146B (See other available formats)
Regulatory Status
RUO
Other Names
Ber-H2, Ki-1, TNFRSF8
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A20146B_PURE_CD30_Antibody_020224
Peripheral blood mononuclear cells were stimulated with anti-CD3/CD28 and recombinant IL-2 (three days) then stained with anti-human CD3 PE and purified anti-human CD30 (clone A20146B) (left) or purified mouse IgG2b, κ isotype control (right) followed by anti-mouse IgG2b APC.
  • A20146B_PURE_CD30_Antibody_020224
    Peripheral blood mononuclear cells were stimulated with anti-CD3/CD28 and recombinant IL-2 (three days) then stained with anti-human CD3 PE and purified anti-human CD30 (clone A20146B) (left) or purified mouse IgG2b, κ isotype control (right) followed by anti-mouse IgG2b APC.
Cat # Size Price Save
306852 100 µg ¥23,100
Description

CD30, also known as Ki-1 antigen, lymphoid activation antigen CD30, and tumor necrosis factor receptor superfamily member 8, is a type I transmembrane receptor that contains four TNF receptor domains with an approximate molecular weight of 64 kD. CD30 is highly expressed on Hodgkins and Reed-Sternberg cells as well as activated, but not resting, T and B cells. CD30 has been shown to interact with a number of proteins including TRAF1, TRAF2, TRAF3, TRAF5, NPM-ALK, TRAF-interacting protein, and CD30 ligand (CD153). Signaling through CD30 is thought to limit the proliferative potential of autoreactive CD8 effector T cells and protect against autoimmunity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human CD30
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3106029 (BioLegend Cat. No. 306852)

Antigen Details

Structure
Type I transmembrane receptor and member of the TNF receptor superfamily that contains 4 TNF receptor domains; predicted molecular weight approximately 64 kD.
Distribution

Highly expressed on Hodgkins and Reed-Sternberg cells and activated, but not resting, T cells and B cells.

Function
Signaling through CD30 is thought to limit the proliferative potential of autoreactive CD8 effector T cells and protect against autoimmunity.
Interaction
TRAF1, TRAF2, TRAF3, TRAF5, NPM-ALK, TRAF-interacting protein, CD30 ligand (CD153)
Ligand/Receptor
CD30 Ligand (CD153)
Cell Type
B cells, Embryonic Stem Cells, Tregs
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Durkop H, et al. 1992. Cell. 68:421-7.
  2. Aizawa S, et al. 1997. J Biol Chem. 272:2042-5.
  3. Stein H, et al. 1982. Int J Cancer. 30:445-59.
Gene ID
943 View all products for this Gene ID
UniProt
View information about CD30 on UniProt.org
Go To Top Version: 1    Revision Date: 02/02/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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