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Purified anti-human CD357 (GITR) Antibody

Pricing & Availability
Clone
621 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Activation-inducible TNFR, AITR, Glucocorticoid-inducible TNFR, TNFRSF18
Isotype
Mouse IgG1, κ
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Product Citations
publications
621_Purified_072307
Human T lymphoma cell line HUT-78 stained with purified 621, followed by biotinylated anti-mouse IgG and Sav-PE
  • 621_Purified_072307
    Human T lymphoma cell line HUT-78 stained with purified 621, followed by biotinylated anti-mouse IgG and Sav-PE
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Cat # Size Price Save
311602 100 µg ¥45,320
Description

GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18). GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and is involved in the development of regulatory T cells and to regulate the activity of Th1 subsets.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 311606).

Application References

(PubMed link indicates BioLegend citation)
  1. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.
  2. Zhou Z, et al. 2008. Proc Natl Acad Sci USA . 105:5465 PubMed
Product Citations
  1. Zhou Z, et al. 2008. Proc Natl Acad Sci U S A. 105:5465. PubMed
  2. Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
  3. Glaczynska M, et al. 2021. In Vivo. 35:283. PubMed
  4. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_314885 (BioLegend Cat. No. 311602)

Antigen Details

Structure
TNFR superfamily, 234-amino acid type I transmembrane protein, 25 kD
Distribution

Activated lymphocytes

Function
Interacts with TRAF's 1-3, but not TRAF 5 or 6, mediates NF-κB activation through the TRAF2/NIK pathway
Ligand/Receptor
GITRL
Cell Type
Lymphocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.

Gene ID
8784 View all products for this Gene ID
UniProt
View information about CD357 on UniProt.org

Related FAQs

I see dim staining with anti human CD357 (GITR) clone 621 in blood and PBMCs.
Our testing has found that anti human CD357 (GITR) clone 621 stains dim with blood and PBMCs and may only be suitable for situations where GITR levels are high, i.e. stimulated cells or specific cell lines expressing high levels of GITR.
Go To Top Version: 2    Revision Date: 08/30/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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