- Clone
- 9E8 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- DCIR, LLIR, CLECSF6, CD367
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![9E8_Purified_CLEC4A_Antibody_FC_083112 9E8_Purified_CLEC4A_Antibody_FC_083112](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9E8_Purified_CLEC4A_Antibody_FC_083112.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human monocyte-derived dendritic cells were stained with purified anti-human CD367 (CLEC4A, clone 9E8) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by biotinylated anti-mouse IgG and Sav-PE.
CD367 (CLEC4A), also known as lectin-like immunoreceptor (LLIR) and dendritic cell immunoreceptor (DCIR), is a type II transmembrane protein belonging to C-type lectin domain family, and was previously designated as CLECSF6. It contains one carbohydrate recognition domain in its C-terminal extracellular domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. CLEC4A is mainly expressed on monocytes, macrophages, dendritic cells, neutrophils, and B cells. It is also found on the surface of CD4+ T cells in rheumatoid arthritis (RA) patients. CLEC4A has been reported to function as a receptor for HIV, cross-present and cross-prime human CD8+ T cells. It might also function as a negative player by recruiting the SHP-1 or/and SHP-2 phosphotase through its immunoreceptor tyrosine-based inhibitory motif (ITIM).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Ectodomain of CLEC4A-human Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2561529 (BioLegend Cat. No. 355302)
Antigen Details
- Structure
- Type II transmembrane protein with single C-type lectin domain, contain immunoreceptor tyrosine-based inhibitory motif (ITIM)
- Distribution
-
Neturophils, monocytes, dendritic cells and B cells
- Function
- Receptor for HIV, cross-presentation and cross-priming of human CD8+ T cells, inhibit intracellular signaling
- Cell Type
- Neutrophils, Monocytes, Dendritic cells, B cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Costimulatory Molecules
- Molecular Family
- CD Molecules, Protein Kinases/Phosphatase
- Antigen References
-
1. Lambert AA, et al. 2011. Blood 117:6589.
2. Klechevsky E, et al. 2010. Blood 116:1685.
3. Kanazawa N, et al. 2002. J. Invest Dermatol. 118:261.
4. Voehringer D, et al. 2001. J. Immunol. 167:4838. - Gene ID
- 50856 View all products for this Gene ID
- UniProt
- View information about CLEC4A on UniProt.org
Related FAQs
Other Formats
View All CLEC4A Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD367 (CLEC4A) | 9E8 | FC |
PE anti-human CD367 (CLEC4A) | 9E8 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD367 (CLEC4A)
Human monocyte-derived dendritic cells were stained with pur... -
PE anti-human CD367 (CLEC4A)
Human monocytes-derived dendritic cells were stained with an...
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