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- Clone
- LN1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1), B cell antigen CD75
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes stained with purified LN1, followed by anti-mouse Igs FITC
CD75 is a 43-85 kD type II transmembrane sialyltransferase protein, also known as beta-galactoside alpha-2,6-sialyltransferase. It is a member of glycosyltransferase family that transfers sialic acid from a donor substrate (CMP-sialic acid) to terminal galactose on glycan chains. CD75 is predominately expressed on germinal center originated B cells and lymphomas. It is also found on mature sIg+ B cells and epithelial cells of lung, breast, kidney, and prostate.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human PBL nuclei
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P, IHC-F, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of frozen sections and formalin-fixed paraffin-embedded sections, and Western blotting3.
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Application References
(PubMed link indicates BioLegend citation) -
- Epstein AL, et al.1984. J. Immunol. 133:1028. (IHC)
- Marder RJ, et al. 1985. Lab. Invest. 52:497. (IHC)
- Kramer G, et al. 2004. BJU Int. 93:822. (WB)
- Product Citations
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- RRID
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AB_2194435 (BioLegend Cat. No. 326901)
Antigen Details
- Structure
- 43-85 kD type II transmembrane sialoprotein, glycosyltransferase family
- Distribution
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Germinal center derived B cells, epithelial cells
- Cell Type
- B cells, Epithelial cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Epstein AL, et al. 1984. J. Immunol. 133:1028.
2. Kramer G, et al. 2004. BJU Int. 93:822.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication - Gene ID
- 6480 View all products for this Gene ID
- UniProt
- View information about CD75 on UniProt.org
Other Formats
View All CD75 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD75 | LN1 | FC,IHC-P,IHC-F,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD75
Human peripheral blood lymphocytes stained with purified LN1...
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