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- Clone
- QA20A40 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lyt3, Ly3, P37, LEU2, CD8B1 CD8b, CD8beta
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with purified anti-human CD8b recombinant (clone QA20A40) (left) or mouse IgG2a, κ isotype control (right) followed by PE goat-anti-mouse IgG. Cells were costained with anti-human CD3 FITC.
CD8b or CD8β antigen is also known as T8, Lyt2, Ly-2, and CD8β. It is a member of the immunoglobulin superfamily expressed on most thymocytes, and some mature T cell subsets. CD8β forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Based on in-house testing, clone QA20A40 reacts with an epitope formed by a heterodimer of human CD8a and CD8b chains, while clone S21011A reacts to an epitope exclusively present on human CD8b.
- RRID
-
AB_2904408 (BioLegend Cat. No. 376702)
Antigen Details
- Structure
- Ig superfamily
- Distribution
-
Most thymocytes, subset of mature T cells
- Function
- Co-receptor for TCR
- Interaction
- CD8α, CD3δ, Lck
- Ligand/Receptor
- MHC class I molecule
- Cell Type
- Lymphocytes, T cells, Thymocytes
- Biology Area
- Adaptive Immunity, Cell Biology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Johnson P. 1987. Immunogenetics. 26:174-7.
- Norment AM & Littman DR, 1988. EMBO J. 7:3433-9.
- Gene ID
- 926 View all products for this Gene ID
- UniProt
- View information about CD8beta on UniProt.org
Related FAQs
Other Formats
View All CD8b Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD8b Recombinant Antibody | QA20A40 | FC |
| Purified anti-human CD8b Recombinant Antibody | QA20A40 | FC |
| APC anti-human CD8b Recombinant Antibody | QA20A40 | FC |
| PE/Cyanine7 anti-human CD8b Recombinant Antibody | QA20A40 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD8b Recombinant Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Purified anti-human CD8b Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD8b Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine7 anti-human CD8b Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu...
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