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- Clone
- EOS9.1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Fas, APO-1, TNFRSF6
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes stained with purified EOS9.1, followed by anti-mouse IgM PE
CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis, and has been shown to play a role in the maintenance of peripheral tolerance. The EOS9.1 antibody is useful for inducing apoptosis of Fas-positive cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis. The Ultra-LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305705 & 305706).
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Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
- Product Citations
-
- RRID
-
AB_314554 (BioLegend Cat. No. 305702)
Antigen Details
- Structure
- TNFR superfamily, type I transmembrane protein, 45 kD
- Distribution
-
T cells and B cells, monocytes, neutrophils, expression level upregulated on activated lymphocytes
- Function
- Mediates apoptosis
- Ligand/Receptor
- Fas ligand (CD178)
- Cell Type
- B cells, Lymphocytes, Monocytes, Neutrophils, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449. - Gene ID
- 355 View all products for this Gene ID
- UniProt
- View information about CD95 on UniProt.org
Other Formats
View All CD95 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD95 (Fas) | EOS9.1 | FC |
| Ultra-LEAF™ Purified anti-human CD95 (Fas) | EOS9.1 | FC,Apop |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD95 (Fas)
Human peripheral blood lymphocytes stained with purified EOS... -
Ultra-LEAF™ Purified anti-human CD95 (Fas)
Human T-cell leukemia cell line, Jurkat, was stimulated (5 h... 
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