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- Clone
- A21043C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
HeLa cells were stained with purified anti-human EGFR (clone A21043C) (filled histogram) or purified mouse lgG1, κ isotype control (open histogram), followed by anti-mouse lgG APC.
Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant fragment of EGFR, aminoacids 25-138
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2936615 (BioLegend Cat. No. 386302)
Antigen Details
- Structure
- Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation
- Distribution
-
Epithelial cells and endothelial cells; frequently found in epithelial carcinomas
- Function
- Controls cell growth and differentiation
- Ligand/Receptor
- Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor
- Cell Type
- Endothelial cells, Epithelial cells
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
- Molecular Family
- CD Molecules, Growth Factors
- Antigen References
-
- da Cunha Santos G, et al. 2011. Annu Rev Pathol. 6:49-69.
- Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522-7.
- Mano M and Humblet Y. 2008. Nat Clin Pract Oncol. 5:415-25.
- Pao W and Chmielecki J. 2010. Nat Rev Cancer. 10:760-74.
- Gene ID
- 1956 View all products for this Gene ID
- UniProt
- View information about EGFR on UniProt.org
Related FAQs
Other Formats
View All EGFR Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human EGFR | A21043C | FC |
| PE anti-human EGFR | A21043C | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human EGFR
HeLa cells were stained with purified anti-human EGFR (clone... -
PE anti-human EGFR
Human cervical cancer cell line HELA was stained with anti-h...
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