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Purified anti-human FCGR2A/CD32A Antibody

Pricing & Availability
Clone
S20004A (See other available formats)
Regulatory Status
RUO
Other Names
FcγRIIa
Isotype
Mouse IgG1, κ
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Product Citations
publications
S20004A_PURE_FCGR2A_CD32A_122821
Human peripheral blood monocytes stained with purified anti-human FCGR2A/CD32A (clone S20004A) (filled histogram), or purified mouse IgG1, κ isotype (open histogram), followed by goat anti-mouse IgG PE antibody .
  • S20004A_PURE_FCGR2A_CD32A_122821
    Human peripheral blood monocytes stained with purified anti-human FCGR2A/CD32A (clone S20004A) (filled histogram), or purified mouse IgG1, κ isotype (open histogram), followed by goat anti-mouse IgG PE antibody .
Cat # Size Price Save
365502 100 µg ¥77,440
Description

CD32A (FCGR2A) is a 40 kD, type I protein with 2 C2-set domains in the extracellular region and 1 ITAM in the intracellular region. CD32A binds monomeric IgG with low affinity but is very efficient binding immune complexes.  FCGR2A is expressed by myeloid cells such as macrophages, monocytes, neutrophils, eosinophils and dendritic cells and also expressed by epithelial cells, platelets and activated CD4+ T cells. CD32A is involved in phagocytosis and clearing of immune complexes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FCGR2A-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

 This clone does not recognizes CD32B-transfected cells

RRID
AB_2910410 (BioLegend Cat. No. 365502)

Antigen Details

Structure
Member of the Ig superfamily, 40 kD, type I protein, 2 C2-set domains, 1 ITAM
Distribution

Macrophages, monocytes, neutrophils, eosinophil, platelets, and CD4+ T Cells

Function
Phagocytosis, cytotoxicity, platelet aggregation
Ligand/Receptor
Aggregated IgG, immune complexes
Cell Type
Eosinophils, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets
Biology Area
Adaptive Immunity, Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Hubbard JJ. et al. 2020. J Exp Med. 217:e20200359.
  2. Newling M, et al. 2020. Clin Exp Immunol. 199:39-49.
  3. Maria H, et al. 2018. Front Immunol. 9:2814.
  4. Descours B, et al. 2017. Nature. 543:564 -567

 

Gene ID
NA
UniProt
View information about FCGR2A/CD32A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/28/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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