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Purified anti-human HB-EGF Antibody

Pricing & Availability
Clone
3H4 (See other available formats)
Regulatory Status
RUO
Other Names
Heparin-binding EGF-like growth factor, HB-EGF-like growth factor
Isotype
Mouse IgG1, κ
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Product Citations
publications
3H4_PURE_HB-EGF_Antibody_082622
NUGC-3 cells (human gastric cancer cells) were stained with purified anti-human HB-EGF (clone 3H4) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
  • 3H4_PURE_HB-EGF_Antibody_082622
    NUGC-3 cells (human gastric cancer cells) were stained with purified anti-human HB-EGF (clone 3H4) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
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Cat # Size Price Save
383702 100 µg ¥84,700
Description

Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of growth factors. Human HB-EGF was initially identified as a protein of 22 kD secreted by macrophage-like U937 cells. It belongs to the EGF family of proteins that includes EGF, TGF-α, HB-EGF, epigen, epiregulin, betacellulin, neuroregulin, and tomoregulin. All the EGF family members are synthesized as type I membrane protein precursors, which can undergo proteolytic cleavage at the plasma membrane to release a mature soluble ectodomain. It has been suggested that various metalloproteinases participate in the shedding of HB-EGF, such as MMP-3, MMP-7, ADAM9, ADAM10, ADAM12, and ADAM17. The ectodomain shedding is stimulated by phorbol esters, calcium ionophore, lysophosphatidic acid, and IL-1β. In addition, nardilysin, a metalloendopeptidase of the M16 family, binds HB-EGF and enhances its shedding through activation of TACE (ADAM17). The membrane-anchored HB-EGF acts in cell-cell and cell-extracellular matrix interactions. In addition, the membrane HB-EGF is the receptor for diphtheria toxin that can be internalized and induce apoptotic death. HB-EGF plays a crucial role in cardiac valvulogenesis. Newborn knockout mice have malformed semilunar and atrioventricular heart valves and poorly differentiated lungs. HB-EGF is overexpressed in several types of cancer such as pancreatic carcinoma, ovarian cancer, and gastric carcinoma. HB-EGF is also involved in cancer metastasis and invasion of ovarian cancer, head and neck cancer, and thyroid carcinoma cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human HB-EGF, extracellular domain (recombinant)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, WB, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hamaoka M, et al. 2010. J Biochem. 148:55.
RRID
AB_2927862 (BioLegend Cat. No. 383702)

Antigen Details

Distribution

Monocytes, macrophages, T cells, neutrophils, eosinophils, myeloid leukemia blasts, myeloma cells, vascular smooth muscle cells, endothelial cells, and normal or neoplastic epithelial cells

Function
HB-EGF is associated with proliferation and differentiation of stromal cells, apoptosis, and morphogenesis. It participates in inflammation, wound healing, atherosclerotic plaque progression, and cardiac valvulogenesis. TNF-α and IL-1β induces HB-EGF in endothelial cells.
Interaction
Fibroblast, smooth muscle cells, monocytes/macrophages, endothelial cells, astrocytes, keratinocytes, normal and neoplastic epithelial cells
Ligand/Receptor
EGFR (ErbB1) and ErbB4; also binds to heparan-sulphate proteoglycans
Cell Type
Epithelial cells, Macrophages, T cells
Biology Area
Angiogenesis, Cancer Biomarkers
Molecular Family
Growth Factors
Antigen References
  1. Higashiyama S, et al. 1991. Science. 251:936.
  2. Hirata M, et al. 2001. Biochem. Biophys. Res. Commun. 283:915.
  3. Jackson LF, et al. 2003. EMBO J. 22:2704.
  4. Nishi E, et al. 2006. J. Biol. Chem. 281:31164.
  5. Yotsumoto F, et al. 2008. Biochem. Biophys. Res. Commun. 365:555.
  6. Ota I, et al. 2013. Oncol Rep. 30:1593.
Gene ID
1839 View all products for this Gene ID
UniProt
View information about HB-EGF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/26/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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