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- Clone
- DT-9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HLAC, MHC Class I Antigen C
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes were stained with purified HLA-C antibody (clone DT-9, filled histogram) or Mouse IgG2b, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
HLA-C belongs to the HLA class I heavy chain paralogues. MHC class I heavy chains associate with β2-microglobulin and are expressed on nearly all nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and thereby play an important role in cell-mediated immune responses and immune surveillance. Detailed characterization of the DT-9 clone indicates that it cross-reacts with HLA-E and certain HLA-A and HLA-B allotypes.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified tamarin MHC class I molecules, corresponding to human HLA-C
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Corrah et al. 2011. J. Virol. 85: 3367.
- Apps et al. 2013. Science. 340:87.
- Braud et al. 1998. Curr. Biol. 8:1.
- Thomas R, et al. 2009. Nat. Genet. 41:1290. (HLA-A/B allotype cross-reactivity)
- Product Citations
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- RRID
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AB_2650941 (BioLegend Cat. No. 373302)
Antigen Details
- Structure
- Ig superfamily
- Distribution
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Nearly all cells
- Function
- Antigen peptide presentation
- Ligand/Receptor
- TCR, CD8
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Gene ID
- 3107 View all products for this Gene ID
- UniProt
- View information about HLA-C on UniProt.org
Related FAQs
Other Formats
View All HLA-C Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human HLA-C | DT-9 | FC |
| Alexa Fluor® 647 anti-human HLA-C | DT-9 | FC |
| Biotin anti-human HLA-C | DT-9 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human HLA-C
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 647 anti-human HLA-C
Human peripheral blood lymphocytes were stained with anti-hu... -
Biotin anti-human HLA-C
Human peripheral blood lymphocytes were stained with anti-hu...
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