- Clone
- MHK-49 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin light chain kappa
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MHK-49_Purified_Igkappa_Cytof_061014.jpg MHK-49_Purified_Igkappa_Cytof_061014.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MHK-49_Purified_Igkappa_Cytof_061014.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human PBMCs stained with 142Nd-anti-CD19 (HIB19) and 160Gd-anti-Ig kappa (MHK-49). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.
The MHK-49 antibody reacts with both soluble and membrane human immunoglobulin light chain kappa (κ). It does not react with human immunoglobulin light chain lambda (λ) or heavy chain. The MHK-49 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Lockridge JL, et al. 2013. Biol. Blood Marrow Transplant 9:1310-22. (ELISA)
- RRID
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AB_2563772 (BioLegend Cat. No. 316527)
Antigen Details
- Structure
- Ig family
- Distribution
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B cells
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 3514 View all products for this Gene ID
- UniProt
- View information about Ig light chain kappa on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
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We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
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The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All Ig light chain κ Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Ig light chain κ
Over night cultured human peripheral blood lymphocytes stain... -
Biotin anti-human Ig light chain κ
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human Ig light chain κ
Human peripheral blood lymphocytes stained with MHK-49 FITC -
PE anti-human Ig light chain κ
CD19+ human B-lymphocytes stained with MHL-38 (an... -
APC anti-human Ig light chain κ
Human peripheral blood mononuclear cells stained with MHK-49... -
Alexa Fluor® 488 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Alexa Fluor® 647 anti-human Ig light chain κ
Human peripheral blood lymphocytes stained with MHK-49 Alexa... -
PerCP/Cyanine5.5 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Pacific Blue™ anti-human Ig light chain κ
Human peripheral blood lymphocytes were stained with CD19 AP... -
PE/Cyanine7 anti-human Ig light chain κ
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APC/Cyanine7 anti-human Ig light chain κ
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Brilliant Violet 421™ anti-human Ig light chain κ
Human peripheral blood lymphocytes were stained with CD19 PE... -
Alexa Fluor® 700 anti-human Ig light chain κ
Human peripheral blood lymphocytes were stained with CD19 FI... -
Purified anti-human Ig light chain κ (Maxpar® Ready)
Human PBMCs stained with 142Nd-anti-CD19 (HIB19) and 160Gd-a... -
APC/Fire™ 750 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
TotalSeq™-A0894 anti-human Ig light chain κ
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TotalSeq™-C0894 anti-human Ig light chain κ
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TotalSeq™-B0894 anti-human Ig light chain κ
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TotalSeq™-D0894 anti-human Ig light chain κ
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PE/Dazzle™ 594 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Spark Red™ 718 anti-human Ig light chain κ (Flexi-Fluor™)
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PE anti-human Ig light chain κ
Typical results from overnight cultured human peripheral blo... -
FITC anti-human Ig light chain κ
Typical results from overnight cultured human peripheral blo... -
Pacific Blue™ anti-human Ig light chain κ
Typical results from overnight cultured human peripheral blo... -
APC anti-human Ig light chain κ
Typical results from overnight cultured human peripheral blo...
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