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- Clone
- Poly5300 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-31,
- Isotype
- Goat Polyclonal Ig
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
This standard curve was generated at BioLegend for demonstration purposes only. Overnight incubation for standard and samples at 4°C is recommended. For detection antibody, two hour incubation with shaking is recommended.
IL-31, produced mainly by activated CD4+ T cells, is a newly discovered helical cytokine belonging to the gp130/IL6 cytokine family. IL-31 is closely related in structure to IL-6, IL-11, and IL-27. Binding of IL-31 to its receptor activates JAK/STAT, PI3K/AKT, and MAPK pathways. IL-31 acts on a broad range of immune and non-immune cells and thus possesses potential pleiotropic physiological functions, such as regulating hematopoiesis and immune responses. Human IL-31 is also involved in inflammatory bowel disease, airway hypersensitivity, and dermatitis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant human IL-31
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-31 recombinant protein ranging from 2000 to 31.3 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
- Application Notes
-
The purified Poly5300 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5300 antibody as the detecting antibody.
- RRID
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AB_2650707 (BioLegend Cat. No. 530002)
Antigen Details
- Structure
- 161 aa with a putative 23 aa signal peptide that is cleaved to generate a 138 aa mature protein.
- Distribution
-
Secreted by activated CD4<sup>+</sup> T cells.
- Function
- IL-31 belongs to the IL-6 family of cytokines and plays a role in skin inflammation.
- Ligand/Receptor
- Binds to IL-31 receptor complex that consists of two receptor subunits, IL31RA and OSMR.
- Cell Type
- T cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. McCandless EE, et al. 2014. Vet. Immunol. Immunopathol. 157:42.
2. Arai I, et al. 2013. Exp. Dermatol. 22:669.
3. Dai X, et al. 2013. PLoS One. 8:e67666.
4. Stott B, et al. 2013. J. Allergy Clin. Immunol. 132:446.
5. Hartmann K, et al. 2013. J. Allergy Clin. Immunol. 132:232.
6. Baron JM and Lüscher B. 2012. Acta. Derm. Venereol. 92:5. - Gene ID
- 386653 View all products for this Gene ID
- UniProt
- View information about IL-31 on UniProt.org
Other Formats
View All IL-31 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IL-31 | Poly5300 | ELISA Capture |
| Biotin anti-human IL-31 | Poly5300 | ELISA Detection |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IL-31
This standard curve was generated at BioLegend for demonstra... -
Biotin anti-human IL-31
This standard curve was generated at BioLegend for demonstra...
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